CXCL14 ELISA Kit

Produktnummer ABIN1889427

Dieses Human CXCL14 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human CXCL14 in Cell Culture Supernatant, Serum, Plasma (heparin) und Plasma (EDTA) zu quantifizieren.

Kurzübersicht für CXCL14 ELISA Kit (ABIN1889427)

Target: CXCL14 (Chemokine (C-X-C Motif) Ligand 14 (CXCL14))

Bindungsspezifität: AA 35-111

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 62.5-4000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Produktdetails CXCL14 ELISA Kit

Untere Nachweisgrenze: 62.5 pg/mL

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CXCL14

Marke: PicoKine™

Analytische Methode: Quantitative

Spezifität: Expression system for standard: E.coli
Immunogen sequence: S35-E111

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Homologie: Bovine,Hamster,Horse,Monkey

Sensitivität: <10pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: E.coli
Immunogen sequence: S35-E111

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Kommentare:

Sequence similarities: Belongs to the intercrine alpha (chemokine CxC) family.

Tissue Specificity: Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highly expressed in normal tissue without inflammatory stimuli and infrequently expressed in cancer cell lines. Weakly expressed in monocyte- derived dendritic cells. Not detected in lung or unstimulated peripheral blood lymphocytes. .

Plattentyp: Pre-coated

Protokoll: human CXCL14 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CXCL14 has been precoated onto 96-well plates. Standards(E.coli, S35-E111) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CXCL14 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CXCL14 amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human CXCL14 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human CXCL14 standard solution and each sample be measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 987, Standard deviation: 35.5, CV(%): 3.6
• Sample 2: n=16, Mean(pg/ml): 1638, Standard deviation: 68.8, CV(%): 4.2
• Sample 3: n=16, Mean(pg/ml): 3285, Standard deviation: 187.2, CV(%): 5.7,
• Sample 1: n=24, Mean(pg/ml): 1387, Standard deviation: 56.87, CV(%): 4.1
• Sample 2: n=24, Mean(pg/ml): 2694, Standard deviation: 156, CV(%): 5.8
• Sample 3: n=24, Mean(pg/ml): 3525, Standard deviation: 225.6, CV(%): 6.4

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Details zu CXCL14

Target: CXCL14 (Chemokine (C-X-C Motif) Ligand 14 (CXCL14))

Andere Bezeichnung: CXCL14

Hintergrund:

Protein Function: Potent chemoattractant for neutrophils, and weaker for dendritic cells. Not chemotactic for T-cells, B-cells, monocytes, natural killer cells or granulocytes. Does not inhibit proliferation of myeloid progenitors in colony formation assays. .

Background: Chemokine(C-X-C motif) ligand 14(CXCL14) is a small cytokine belonging to the CXC chemokine family that is also known as BRAK(for breast and kidney-expressed chemokine). This gene is mapped to 5q31.1. CXCL14 is constitutively expressed at high levels in many normal tissues, it has been found that fibroblasts are the primary source of CXCL14 and that CXCL14 is involved in the homeostasis of monocyte-derived macrophages rather than in inflammation. It is also a potent chemoattractant and activator of dendritic cells, is implicated in homing of these cells, and can stimulate the migration of activated NK cells. CXCL14 also inhibits angiogenesis, possibly as a result of its ability to block endothelial cell chemotaxis. In addition to it, CXCL14 is a potent chemokine for immature DCs, with a chemoattractive activity comparable to bacterial fMLP and MIP1A.

Synonyms: C-X-C motif chemokine 14,Chemokine BRAK,MIP-2G,Small-inducible cytokine B14,CXCL14,MIP2G, NJAC, SCYB14,PSEC0212, UNQ240/PRO273,

Full Gene Name: C-X-C motif chemokine 14

Cellular Localisation: Secreted.

Gen-ID: 9547

UniProt: O95715

Pathways: Autophagie