CRISP3 ELISA Kit

Produktnummer ABIN1889426

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human CRISP3.

Kurzübersicht für CRISP3 ELISA Kit (ABIN1889426)

Target: CRISP3 (Cysteine-Rich Secretory Protein 3 (CRISP3))

Bindungsspezifität: AA 21-245

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 1.56-100 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Saliva, Urine, Tissue Homogenate

Produktdetails CRISP3 ELISA Kit

Untere Nachweisgrenze: 1.56 ng/mL

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CRISP3

Marke: PicoKine™

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NSO
Immunogen sequence: N21-Y245

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <50pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: N21-Y245

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Kommentare:

Sequence similarities: Belongs to the CRISP family.

Tissue Specificity: Salivary gland, pancreas and prostate > epididymis, ovary, thymus and colon.

Plattentyp: Pre-coated

Protokoll: human CRISP3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CRISP3 has been precoated onto 96-well plates. Standards(NSO, N21-Y245) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CRISP3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CRISP3 amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 100 ng/mL, 50 ng/mL, 25 ng/mL, 1.25 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL human CRISP3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma (heparin, EDTA), saliva, urine or tissue homogenates to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human CRISP3 standard solution and each sample is measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(ng/ml): 18, Standard deviation: 0.63, CV(%): 3.5
• Sample 2: n=16, Mean(ng/ml): 41, Standard deviation: 1.7, CV(%): 4.1
• Sample 3: n=16, Mean(ng/ml): 72, Standard deviation: 2.74, CV(%): 3.8,
• Sample 1: n=24, Mean(ng/ml): 20, Standard deviation: 1.14, CV(%): 5.7
• Sample 2: n=24, Mean(ng/ml): 38, Standard deviation: 2.4, CV(%): 6.3
• Sample 3: n=24, Mean(ng/ml): 65, Standard deviation: 3.8, CV(%): 5.8

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Details zu CRISP3

Target: CRISP3 (Cysteine-Rich Secretory Protein 3 (CRISP3))

Andere Bezeichnung: CRISP3

Hintergrund:

Background: Cysteine-rich secretory protein 3 is a cysteine-rich secretory protein that in humans is encoded by the CRISP3 gene. CRISP3 expression is found in the secretory epithelium throughout the male genital tract, with particularly high expression in the cauda epididymis and ampulla vas deferens. It is a potential biomarker for prostate cancer. CRISP3 gene can be defined as an early-transcribed gene with a putative role in the pathophysiology of sjogren's syndrome. CRISP3 is present in exocrine secretions and in secretory granules of neutrophilic granulocytes and is believed to play a role in innate immunity.

Synonyms: Cysteine-rich secretory protein 3,CRISP-3,Specific granule protein of 28 kDa,SGP28,CRISP3,

Full Gene Name: Cysteine-rich secretory protein 3

Cellular Localisation: Secreted. In neutrophils, localized in specific granules.

Gen-ID: 10321

UniProt: P54108