CRISP3 ELISA Kit

Produktnummer ABIN6720320

Hersteller Produkt- Nr.: koa0760

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human CRISP3.

Kurzübersicht für CRISP3 ELISA Kit (ABIN6720320)

Target: CRISP3 (Cysteine-Rich Secretory Protein 3 (CRISP3))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 62.5 pg/mL - 4000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Saliva, Serum, Urine

Produktdetails CRISP3 ELISA Kit

Untere Nachweisgrenze: 62.5 pg/mL

Hersteller Produkt- Nr.: koa0760

Hersteller: Rockland

Verwendungszweck: Human CRISP3 Sandwich ELISA Kit for Quantitative Detection

Marke: AccuSignal™

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NS0
Immunogen sequence: N21-Y245

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 50 pg/mL

Bestandteile:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Benötigtes Material: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Anwendungsinformationen

Kommentare:

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 100 ng/mL, 50 ng/mL, 25 ng/mL, 1.25 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL human CRISP3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma (heparin, EDTA), saliva, urine or tissue homogenates to each empty well. We recommend that each human CRISP3 standard solution and each sample is measured in duplicate.

Testpräzision: Intra-Assay Precision (CV%): 4.1%, 6.2%, 4.5%
Inter-Assay Precision (CV%): 4.7%, 6.4%, 5.4%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu CRISP3

Target: CRISP3 (Cysteine-Rich Secretory Protein 3 (CRISP3))

Andere Bezeichnung: CRISP3

Hintergrund:

Background: Cysteine-rich secretory protein 3 is a cysteine-rich secretory protein that in humans is encoded by the CRISP3 gene. CRISP3 expression is found in the secretory epithelium throughout the male genital tract, with particularly high expression in the cauda epididymis and ampulla vas deferens. It is a potential biomarker for prostate cancer. CRISP3 gene can be defined as an early-transcribed gene with a putative role in the pathophysiology of sjogren's syndrome. CRISP3 is present in exocrine secretions and in secretory granules of neutrophilic granulocytes and is believed to play a role in innate immunity.

Gene Full Name: cysteine rich secretory protein 3

Gen-ID: 10321

NCBI Accession: NP_001177915

UniProt: P54108