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FGF1 ELISA Kit (Fibroblast Growth Factor 1 (Acidic)) ELISA Kit

FGF1 Reaktivität: Ratte AA 16-155 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Produktnummer ABIN1889350
$369.00
Zzgl. Versandkosten $45.00
96 tests
Lieferung in 4 bis 6 Werktagen
  • Target Alle FGF1 ELISA Kits anzeigen
    FGF1
    Bindungsspezifität
    AA 16-155
    Reaktivität
    • 6
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    Ratte
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    31.2-2000 pg/mL
    Untere Nachweisgrenze
    31.2 pg/mL
    Applikation
    ELISA
    Verwendungszweck
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat FGF1
    Marke
    PicoKine™
    Proben
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytische Methode
    Quantitative
    Spezifität
    Expression system for standard: E.coli
    Immunogen sequence: F16-D155
    Kreuzreaktivität (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivität
    <10pg/mL
    Benötigtes Material
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: E.coli
    Immunogen sequence: F16-D155
    Featured
    Zu unserem meistverkauften FGF1 ELISA Kit
  • Applikationshinweise
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Plattentyp
    Pre-coated
    Protokoll
    rat FGF1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for FGF1 has been precoated onto 96-well plates. Standards(E.coli, F16-D155) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FGF1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat FGF1 amount of sample captured in plate.
    Testdurchführung

    Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL rat FGF1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each rat FGF1 standard solution and each sample is measured in duplicate.

    Testpräzision
    • Sample 1: n=16, Mean(pg/ml): 156, Standard deviation: 7.49, CV(%): 4.8
    • Sample 2: n=16, Mean(pg/ml): 758, Standard deviation: 56.85, CV(%): 7.5
    • Sample 3: n=16, Mean(pg/ml): 1229, Standard deviation: 82.34, CV(%): 6.7,
    • Sample 1: n=24, Mean(pg/ml): 157, Standard deviation: 13.82, CV(%): 8.8
    • Sample 2: n=24, Mean(pg/ml): 749, Standard deviation: 65.16, CV(%): 8.7
    • Sample 3: n=24, Mean(pg/ml): 1226, Standard deviation: 91.95, CV(%): 7.5
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    Avoid multiple freeze-thaw cycles.
    Lagerung
    -20 °C,4 °C
    Informationen zur Lagerung
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Haltbarkeit
    12 months
  • Target Alle FGF1 ELISA Kits anzeigen
    FGF1
    Andere Bezeichnung
    FGF1 (FGF1 Produkte)
    Synonyme
    AFGF, ECGF, ECGF-beta, ECGFA, ECGFB, FGF-1, FGF-alpha, FGFA, GLIO703, HBGF-1, HBGF1, Fgf1, FGF1, aFGF, ecgf, fgfa, ecgfa, ecgfb, fgf-1, hbgf1, glio703, ecgf-beta, fgf-alpha, Dffrx, Fam, Fgf-1, Fgfa, zgc:136885, LOC100221393, fibroblast growth factor 1, fibroblast growth factor 1 L homeolog, fibroblast growth factor 1b, FGF1, fgf1.L, fgf1, Fgf1, fgf1b, LOC100221393
    Hintergrund

    Protein Function: Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro.

    Background: Heparin-binding growth factor 1 is a protein that in humans is encoded by the FGF1 gene. The protein encoded by this gene is a member of the fibroblast growth factor(FGF) family. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. The FGF1 gene was mapped to chromosome 5q31.3-q33.2 by in situ hybridization.

    Synonyms: Fibroblast growth factor 1,FGF-1,Acidic fibroblast growth factor,aFGF,Heparin-binding growth factor 1,HBGF-1,Fgf1,Fgf-1, Fgfa,

    Full Gene Name: Fibroblast growth factor 1

    Cellular Localisation: Secreted . Cytoplasm . Cytoplasm, cell cortex . Cytoplasm, cytosol . Nucleus . Lacks a cleavable signal sequence. Within the cytoplasm, it is transported to the cell membrane and then secreted by a non- classical pathway that requires Cu(2+) ions and S100A13. Secreted in a complex with SYT1 (By similarity). Binding of exogenous FGF1 to FGFR facilitates endocytosis followed by translocation of FGF1 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as LRRC59 (By similarity)..
    Gen-ID
    25317
    UniProt
    P61149
    Pathways
    RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung
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