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FLT3LG ELISA Kit

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human FLT3LG.
Produktnummer ABIN1889291

Kurzübersicht für FLT3LG ELISA Kit (ABIN1889291)

Target

Alle FLT3LG ELISA Kits anzeigen
FLT3LG (Fms-Related tyrosine Kinase 3 Ligand (FLT3LG))

Bindungsspezifität

AA 27-185

Reaktivität

  • 8
  • 6
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Human

Nachweismethode

Colorimetric

Methodentyp

Sandwich ELISA

Detektionsbereich

15.6-1000 pg/mL

Applikation

ELISA

Proben

Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)
  • Untere Nachweisgrenze

    15.6 pg/mL

    Verwendungszweck

    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Flt-3ligand

    Marke

    PicoKine™

    Analytische Methode

    Quantitative

    Spezifität

    Expression system for standard: sf21
    Immunogen sequence: T27-P185

    Kreuzreaktivität (Details)

    There is no detectable cross-reactivity with other relevant proteins.

    Sensitivität

    <10pg/mL

    Benötigtes Material

    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

    Immunogen

    Expression system for standard: sf21
    Immunogen sequence: T27-P185
  • Applikationshinweise

    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

    Plattentyp

    Pre-coated

    Protokoll

    human Flt-3ligand ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Flt-3ligand has been precoated onto 96-well plates. Standards(sf21, T27-P185) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Flt-3ligand is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Flt-3ligand amount of sample captured in plate.

    Testdurchführung

    Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human Flt-3ligand standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Flt-3ligand standard solution and each sample be measured in duplicate.

    Testpräzision

    • Sample 1: n=16, Mean(pg/ml): 109, Standard deviation: 3.49, CV(%): 3.2
    • Sample 2: n=16, Mean(pg/ml): 256, Standard deviation: 8.45, CV(%): 3.3
    • Sample 3: n=16, Mean(pg/ml): 520, Standard deviation: 22.88, CV(%): 4.4,
    • Sample 1: n=24, Mean(pg/ml): 112, Standard deviation: 10.64, CV(%): 9.5
    • Sample 2: n=24, Mean(pg/ml): 245, Standard deviation: 18.38, CV(%): 7.5
    • Sample 3: n=24, Mean(pg/ml): 518, Standard deviation: 41.96, CV(%): 8.1

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Handhabung

    Avoid multiple freeze-thaw cycles.

    Lagerung

    -20 °C,4 °C

    Informationen zur Lagerung

    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

    Haltbarkeit

    12 months
  • Target Alle FLT3LG ELISA Kits anzeigen

    FLT3LG (Fms-Related tyrosine Kinase 3 Ligand (FLT3LG))

    Andere Bezeichnung

    FLT3LG

    Hintergrund

    Protein Function: Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins.

    Background: Fms-related tyrosine kinase 3 ligand(FLT3LG), also known as Flt-3ligand or FL, is a protein which in humans is encoded by the FLT3LG gene. It is mapped to 19q13.3. Flt-3ligand is a hematopoietic four helical bundle cytokine. It is structurally homologous to stem cell factor(SCF) and colony stimulating factor 1(CSF-1). In synergy with other growth factors, Flt-3ligand stimulates the proliferation and differentiation of various blood cell progenitors. Besides that, Flt-3ligand can control the development of Dendritic cells(DCs) and is particularly important for plasmacytoid DCs and CD8-positive classical DCs and their CD103-positive tissue counterparts. Flt-3ligand also can enhance the response of stem and primitive progenitor cells to other growth factors to generate all myeloid lineages except erythroid cells.

    Synonyms: Fms-related tyrosine kinase 3 ligand,Flt3 ligand,Flt3L,SL cytokine,FLT3LG,

    Full Gene Name: Fms-related tyrosine kinase 3 ligand

    Cellular Localisation: Isoform 1: Cell membrane, Single-pass type I membrane protein.

    Gen-ID

    2323

    UniProt

    P49771

    Pathways

    RTK Signalweg
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