SensoLyte® Homogeneous AFC Caspase-3/7 Assay Kit

Produktnummer ABIN1882430

Kurzübersicht für SensoLyte® Homogeneous AFC Caspase-3/7 Assay Kit (ABIN1882430)

Target: Caspase 3 (CASP3)

Nachweismethode: Fluorometric

Applikation: Fluorescence Resonance Energy Transfer Microscopy (FRET)

Produktdetails

Produktmerkmale: The SensoLyte® Homogeneous AFC Capase-3/7 Assay Kit uses Ac-DEVD-AFC as the fluorogenic indicator for assaying caspase-3/7 activities. Upon caspase-3/7 cleavage, Ac-DEVD-AFC generates the AFC fluorophore which has bright blue fluorescence and can be detected at Ex/Em=380 nm/500 nm. A bi-function assay buffer in this kit is designed to lyze the cells and measure the enzyme activity at the same time. Thus, this kit can measure caspase-3/7 activity in cell culture directly in a 96-well or 384-well plate without a time-consuming cell extraction step. In case the cells are cultured in larger plates or flasks, a lysis buffer and protocol for cell lysate preparation are also conveniently included in the kit. The kit is suitable for high throughput screening of apoptosis inducers and inhibitors.

Anwendungsinformationen

Kommentare:

FRET-based Assay Kit

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Protect Component A from light and moisture. Keep Components A and B away from light.

Lagerung: -20 °C

Informationen zur Lagerung: Store Components A, B, C, and E at -20 °C. For convenience, Components D and F can be stored at 4 °C.

Referenzen

Freise, Ruehl, Seehofer, Hoyer, Somasundaram: "The inhibitor of Ca(2+)-dependent K+ channels TRAM-34 blocks growth of hepatocellular carcinoma cells via downregulation of estrogen receptor alpha mRNA and nuclear factor-kappaB." in: Investigational new drugs, Vol. 31, Issue 2, pp. 452-7, (2013) (PubMed).

Meek, LeBoeuf, Saha, Alpers, Hudkins, Cooney, Anderberg, Tuttle: "Glomerular cell death and inflammation with high-protein diet and diabetes." in: Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, Vol. 28, Issue 7, pp. 1711-20, (2013) (PubMed).

Cheung, Revers, Perampalam, Wei, Kiarash, Green, Abdul-Wahid, Gariépy: "An evolved ribosome-inactivating protein targets and kills human melanoma cells in vitro and in vivo." in: Molecular cancer, Vol. 9, pp. 28, (2010) (PubMed).

Freise, Erben, Neuman, Kim, Zeitz, Somasundaram, Ruehl: "An active extract of Lindera obtusiloba inhibits adipogenesis via sustained Wnt signaling and exerts anti-inflammatory effects in the 3T3-L1 preadipocytes." in: The Journal of nutritional biochemistry, Vol. 21, Issue 12, pp. 1170-7, (2010) (PubMed).

Ruehl, Erben, Kim, Freise, Dagdelen, Eisele, Trowitzsch-Kienast, Zeitz, Jia, Stickel, Somasundaram: "Extracts of Lindera obtusiloba induce antifibrotic effects in hepatic stellate cells via suppression of a TGF-beta-mediated profibrotic gene expression pattern." in: The Journal of nutritional biochemistry, Vol. 20, Issue 8, pp. 597-606, (2009) (PubMed).

Antigendetails

Target: Caspase 3 (CASP3)

Andere Bezeichnung: Caspase-3

Hintergrund: Central to the execution phase of apoptosis are the two closely related caspase-3 and caspase-7. They share common substrate specificity and structure, but differ completely in the sequence of their respective N-terminal regions. Both caspase-3 and caspase-7 have substrate selectivity for the amino acid sequence Asp-Glu-Val-Asp (DEVD).