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GDF15 ELISA Kit

Dieses Maus GDF15 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Maus GDF15 in Cell Culture Supernatant, Serum, Plasma (heparin) und Plasma (EDTA) zu quantifizieren.
Produktnummer ABIN1706082

Kurzübersicht für GDF15 ELISA Kit (ABIN1706082)

Target

Alle GDF15 ELISA Kits anzeigen
GDF15 (Growth Differentiation Factor 15 (GDF15))

Bindungsspezifität

AA 189-303

Reaktivität

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Maus

Nachweismethode

Colorimetric

Methodentyp

Sandwich ELISA

Detektionsbereich

15.6-1000 pg/mL

Applikation

ELISA

Proben

Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
  • Untere Nachweisgrenze

    15.6 pg/mL

    Verwendungszweck

    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse GDF-15

    Marke

    PicoKine™

    Analytische Methode

    Quantitative

    Spezifität

    Expression system for standard: NSO
    Immunogen sequence: S189-A303

    Kreuzreaktivität (Details)

    There is no detectable cross-reactivity with other relevant proteins.

    Sensitivität

    <10pg/mL

    Benötigtes Material

    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

    Immunogen

    Expression system for standard: NSO
    Immunogen sequence: S189-A303
  • Applikationshinweise

    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

    Kommentare

    Tissue Specificity: Highly expressed in liver. .

    Plattentyp

    Pre-coated

    Protokoll

    mouse GDF-15 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for GDF-15 has been precoated onto 96-well plates. Standards(NSO, S189-A303) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for GDF-15 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse GDF-15 amount of sample captured in plate.

    Testdurchführung

    Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL mouse GDF-15 standard solutions into the pre-coated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse GDF-15 standard solution and each sample is measured in duplicate.

    Testpräzision

    • Sample 1: n=16, Mean(pg/ml): 114, Standard deviation: 4.33, CV(%): 3.8
    • Sample 2: n=16, Mean(pg/ml): 346, Standard deviation: 12.46, CV(%): 3.6
    • Sample 3: n=16, Mean(pg/ml): 548, Standard deviation: 22.47, CV(%): 4.1,
    • Sample 1: n=24, Mean(pg/ml): 98.2, Standard deviation: 7.37, CV(%): 7.5
    • Sample 2: n=24, Mean(pg/ml): 359, Standard deviation: 20.10, CV(%): 5.6
    • Sample 3: n=24, Mean(pg/ml): 579, Standard deviation: 39.37, CV(%): 6.8

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Handhabung

    Avoid multiple freeze-thaw cycles.

    Lagerung

    -20 °C,4 °C

    Informationen zur Lagerung

    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

    Haltbarkeit

    12 months
  • Target Alle GDF15 ELISA Kits anzeigen

    GDF15 (Growth Differentiation Factor 15 (GDF15))

    Andere Bezeichnung

    GDF15

    Hintergrund

    Background: GDF-15(Growth differentiation factor 15),also known as TGF-PL, MIC-1, PDF, PLAB, and PTGFB, is a protein belonging to the transforming growth factor beta superfamily that has a role in regulating inflammatory and apoptotic pathways in injured tissues and during disease processes. Using FISH, the MIC1 gene is mapped to 19p13.2-p13.1. Its expression in liver can be significantly up-regulated in during injury of organs such as liver, kidney, heart and lung. GDF15 showed increased expression and secretion during erythroblast maturation. GDF15 functions as an antiinflammatory cytokine by directly interfering with chemokine signaling and integrin activation.

    Synonyms: Growth/differentiation factor 15,GDF-15,Gdf15,Sbf,

    Full Gene Name: Growth/differentiation factor 15

    Cellular Localisation: Secreted.

    Gen-ID

    23886

    UniProt

    Q9Z0J7

    Pathways

    SARS-CoV-2 Protein Interaktom
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