GDF15 ELISA Kit

Produktnummer ABIN6720167

Hersteller Produkt- Nr.: koa0453

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human GDF15.

Kurzübersicht für GDF15 ELISA Kit (ABIN6720167)

Target: GDF15 (Growth Differentiation Factor 15 (GDF15))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.2 pg/mL - 2000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum

Produktdetails GDF15 ELISA Kit

Untere Nachweisgrenze: 31.2 pg/mL

Hersteller Produkt- Nr.: koa0453

Hersteller: Rockland

Verwendungszweck: Human GDF15 Sandwich ELISA Kit for Quantitative Detection

Marke: AccuSignal™

Analytische Methode: Quantitative

Spezifität: Expression system for standard: CHO
Immunogen sequence: A197-I308

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 10 pg/mL

Bestandteile:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Benötigtes Material: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Anwendungsinformationen

Kommentare:

Capture antibody and Detection antibody: polyclonal antibody from goat, polyclonal antibody from goat

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human GDF-15 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human GDF-15 standard solution and each sample be measured in duplicate.

Testpräzision: Intra-Assay Precision (CV%): 6.7%, 6.4%, 7%
Inter-Assay Precision (CV%): 7.4%, 7.6%, 7.3%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu GDF15

Target: GDF15 (Growth Differentiation Factor 15 (GDF15))

Andere Bezeichnung: GDF-15

Hintergrund:

Background: GDF-15 (Growth differentiation factor 15),also known as TGF-PL, MIC-1, PDF, PLAB, and PTGFB, is a protein belonging to the transforming growth factor beta superfamily that has a role in regulating inflammatory and apoptotic pathways in injured tissues and during disease processes. Using FISH, the MIC1 gene is mapped to 19p13.2-p13.1. Its expression in liver can be significantly up-regulated in during injury of organs such as liver, kidney, heart and lung. GDF15 showed increased expression and secretion during erythroblast maturation. GDF15 functions as an antiinflammatory cytokine by ly interfering with chemokine signaling and integrin activation.

Gene Full Name: growth differentiation factor 15

Gen-ID: 9518

NCBI Accession: NP_004855

UniProt: Q99988

Pathways: SARS-CoV-2 Protein Interaktom