Neurotrophin 4 ELISA Kit

Produktnummer ABIN1706065

Human Neurotrophin 4 ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human Neurotrophin 4 und wurde in 2+ Publikationen erwähnt.

Kurzübersicht für Neurotrophin 4 ELISA Kit (ABIN1706065)

Target: Neurotrophin 4 (NTF4)

Bindungsspezifität: AA 81-210

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.2-2000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum

Produktdetails Neurotrophin 4 ELISA Kit

Untere Nachweisgrenze: 31.2 pg/mL

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human NT-4

Marke: PicoKine™

Analytische Methode: Quantitative

Spezifität: Expression system for standard: sf21
Immunogen sequence: G81-A210

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <10pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: sf21
Immunogen sequence: G81-A210

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Kommentare:

Tissue Specificity: Highest levels in prostate, lower levels in thymus, placenta, and skeletal muscle. Expressed in embryonic and adult tissues.

Plattentyp: Pre-coated

Protokoll: human NT-4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for NT-4 has been precoated onto 96-well plates. Standards(sf21, G81-A210) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for NT-4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human NT-4 amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human NT-4 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human NT-4 standard solution and each sample be measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 248, Standard deviation: 11.16, CV(%): 4.5
• Sample 2: n=16, Mean(pg/ml): 745, Standard deviation: 38, CV(%): 5.1
• Sample 3: n=16, Mean(pg/ml): 1243, Standard deviation: 78.31, CV(%): 6.3,
• Sample 1: n=24, Mean(pg/ml): 280, Standard deviation: 14.84, CV(%): 5.3
• Sample 2: n=24, Mean(pg/ml): 821, Standard deviation: 46, CV(%): 5.6
• Sample 3: n=24, Mean(pg/ml): 1402, Standard deviation: 96.74, CV(%): 6.9

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Referenzen für Neurotrophin 4 ELISA Kit (ABIN1706065)

Li, Jin, Wang, Li, Liu, Chang, Wang, Liu: "Live attenuated Salmonella displaying HIV-1 10E8 epitope on fimbriae: systemic and mucosal immune responses in BALB/c mice by mucosal administration." in: Scientific reports, Vol. 6, pp. 29556, (2018) (PubMed).

Sun, Zhang, Li, Wang, Ma, Sun, Liu, Wang, Zhou: "Interaction between neurotrophin 4 and gonadotrophin in bovine oviducts." in: Theriogenology, Vol. 78, Issue 1, pp. 39-48, (2012) (PubMed).

Details zu Neurotrophin 4

Target: Neurotrophin 4 (NTF4)

Andere Bezeichnung: NTF4

Hintergrund:

Protein Function: Target-derived survival factor for peripheral sensory sympathetic neurons.

Background: Neurotrophin-4(NT-4), also known as neurotrophin-5(NT-5), is a protein that in humans is encoded by the NTF4 gene. Human NT-4 as well as a human NT-4 pseudogene colocalize to chromosome 19 band q13.3. NT-4 is a member of a family of neurotrophic factors, the neurotrophins, that control survival and differentiation of vertebrate neurons(2-4). NT-4 is a neurotrophic factor that signals predominantly through the TrkB receptor tyrosine kinase. NT4 plays a physiological role essential for hippocampus- and amygdala-dependent long-term memory and hippocampal long-lasting LTP and that NT4 may be useful in the therapy of acquired disorders of learning and memory.

Synonyms: Neurotrophin-4,NT-4,Neurotrophin-5,NT-5,Neutrophic factor 4,NTF4,NTF5,

Full Gene Name: Neurotrophin-4

Cellular Localisation: Secreted.

Gen-ID: 4909

UniProt: P34130

Pathways: RTK Signalweg