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Betacellulin ELISA Kit

This anti-Betacellulin antibody (ABIN1672881) is a antibody detecting Betacellulin in ELISA. Suitable for Human.
Produktnummer ABIN1672881

Kurzübersicht für Betacellulin ELISA Kit (ABIN1672881)

Target

Alle Betacellulin (BTC) ELISA Kits anzeigen
Betacellulin (BTC)

Bindungsspezifität

AA 32-111

Reaktivität

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Human

Nachweismethode

Colorimetric

Methodentyp

Sandwich ELISA

Detektionsbereich

15.6-1000 pg/mL

Applikation

ELISA

Proben

Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
  • Untere Nachweisgrenze

    15.6 pg/mL

    Verwendungszweck

    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Betacellulin/BTC

    Marke

    PicoKine™

    Analytische Methode

    Quantitative

    Spezifität

    Expression system for standard: E.coli
    Immunogen sequence: D32-Y111

    Kreuzreaktivität (Details)

    There is no detectable cross-reactivity with other relevant proteins.

    Sensitivität

    <10pg/mL

    Benötigtes Material

    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

    Immunogen

    Expression system for standard: E.coli
    Immunogen sequence: D32-Y111
  • Applikationshinweise

    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

    Kommentare

    Tissue Specificity: Synthesized in several tissues and tumor cells. Predominantly expressed in pancreas and small intestine. .

    Plattentyp

    Pre-coated

    Protokoll

    human Betacellulin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Betacellulin has been precoated onto 96-well plates. Standards(E.coli, D32-Y111) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Betacellulin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Betacellulin amount of sample captured in plate.

    Testdurchführung

    Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL human Betacellulin standard solutions into the pre-coated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human Betacellulin standard solution and each sample is measured in duplicate.

    Testpräzision

    • Sample 1: n=16, Mean(pg/ml): 46, Standard deviation: 2.35, CV(%): 5.1
    • Sample 2: n=16, Mean(pg/ml): 119, Standard deviation: 6.31, CV(%): 5.3
    • Sample 3: n=16, Mean(pg/ml): 725, Standard deviation: 45.68, CV(%): 6.3,
    • Sample 1: n=24, Mean(pg/ml): 47, Standard deviation: 2.73, CV(%): 5.8
    • Sample 2: n=24, Mean(pg/ml): 124, Standard deviation: 7.69, CV(%): 6.2
    • Sample 3: n=24, Mean(pg/ml): 736, Standard deviation: 58.14, CV(%): 7.9

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Handhabung

    Avoid multiple freeze-thaw cycles.

    Lagerung

    -20 °C,4 °C

    Informationen zur Lagerung

    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

    Haltbarkeit

    12 months
  • Target Alle Betacellulin (BTC) ELISA Kits anzeigen

    Betacellulin (BTC)

    Andere Bezeichnung

    BTC

    Hintergrund

    Protein Function: Growth factor that binds to EGFR, ERBB4 and other EGF receptor family members. Potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells. .

    Background: Betacellulin, also known as BTC is a protein that in humans is encoded by the BTC gene. This gene is mapped to 4q13.3. The protein encoded by this gene is a member of the EGF family of growth factors. It is synthesized primarily as a transmembrane precursor, which is then processed to mature molecule by proteolytic events. This protein is a ligand for the EGF receptor. It is the growth factor that binds to EGFR, ERBB4 and other EGF receptor family members. This protein is a potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells.

    Synonyms: Probetacellulin,Betacellulin,BTC,BTC,

    Full Gene Name: Probetacellulin

    Cellular Localisation: Betacellulin: Secreted, extracellular space.

    Gen-ID

    685

    UniProt

    P35070

    Pathways

    RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung
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