DKK1 ELISA Kit

Produktnummer ABIN1672840

Maus DKK1 ELISA-Kit Colorimetric-Assay zur Quantifizierung von Maus DKK1 und wurde in 1 Publikation erwähnt.

Kurzübersicht für DKK1 ELISA Kit (ABIN1672840)

Target: DKK1 (Dickkopf Homolog 1 (DKK1))

Bindungsspezifität: AA 30-272

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 46.9-3000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Produktdetails DKK1 ELISA Kit

Untere Nachweisgrenze: 46.9 pg/mL

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse DKK1

Marke: PicoKine™

Analytische Methode: Quantitative

Spezifität: Expression system for standard: CHO
Immunogen sequence: S30-H272

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <15pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: CHO
Immunogen sequence: S30-H272

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plattentyp: Pre-coated

Protokoll: mouse DKK1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for DKK1 has been precoated onto 96-well plates. Standards(CHO, S30-H272) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for DKK1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse DKK1 amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 3000pg/mL, 1500pg/mL, 750pg/mL, 375.pg/mL, 187.5pg/mL, 93.8pg/mL, 46.9pg/mL mouse DKK1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernatants, serum or plasma (heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse DKK1 standard solution and each sample is measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 228, Standard deviation: 12.54, CV(%): 5.5
• Sample 2: n=16, Mean(pg/ml): 845, Standard deviation: 56.6, CV(%): 6.7
• Sample 3: n=16, Mean(pg/ml): 1474, Standard deviation: 91.39, CV(%): 6.2,
• Sample 1: n=24, Mean(pg/ml): 259, Standard deviation: 17.1, CV(%): 6.6
• Sample 2: n=24, Mean(pg/ml): 897, Standard deviation: 66.4, CV(%): 7.4
• Sample 3: n=24, Mean(pg/ml): 1532, Standard deviation: 105.7, CV(%): 6.9

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Referenzen für DKK1 ELISA Kit (ABIN1672840)

Sugatani, Agapova, Malluche, Hruska: "SIRT6 deficiency culminates in low-turnover osteopenia." in: Bone, Vol. 81, pp. 168-77, (2015) (PubMed).

Details zu DKK1

Target: DKK1 (Dickkopf Homolog 1 (DKK1))

Andere Bezeichnung: DKK1

Hintergrund:

Protein Function: Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. .

Background: Dickkopf-related protein 1 is a protein that in humans is encoded by the DKK1 gene, which was mapped to chromosome 10q11.2. This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow, plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.

Synonyms: Dickkopf-related protein 1,Dickkopf-1,Dkk-1,mDkk-1,Dkk1,

Full Gene Name: Dickkopf-related protein 1

Cellular Localisation: Secreted.

Gen-ID: 13380

UniProt: O54908

Pathways: WNT Signalweg, Regulation of Muscle Cell Differentiation, Positive Regulation of fat Cell Differentiation