CD25 ELISA Kit

Produktnummer ABIN1672833

Dieses Maus CD25 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Maus CD25 zu quantifizieren.

Kurzübersicht für CD25 ELISA Kit (ABIN1672833)

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Bindungsspezifität: AA 22-236

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 78-5000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Produktdetails CD25 ELISA Kit

Untere Nachweisgrenze: 78 pg/mL

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse CD25/IL-2sR alpha

Marke: PicoKine™

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NSO,E22-K236

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <5pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: E22-K236

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plattentyp: Pre-coated

Protokoll: mouse CD25 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD25 has been precoated onto 96-well plates. Standards (NSO,E22-K236) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD25 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD25 amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 313pg/mL, 156pg/mL, 78pg/mL mouse CD25 standard solutions into the pre-coated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma( heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse CD25 standard solution and each sample is measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 564, Standard deviation: 32.7, CV(%): 5.8
• Sample 2: n=16, Mean(pg/ml): 1244, Standard deviation: 75.9, CV(%): 6.1
• Sample 3: n=16, Mean(pg/ml): 2860, Standard deviation: 157.3, CV(%): 5.5,
• Sample 1: n=24, Mean(pg/ml): 758, Standard deviation: 54.6, CV(%): 7.2
• Sample 2: n=24, Mean(pg/ml): 1654, Standard deviation: 134, CV(%): 8.1
• Sample 3: n=24, Mean(pg/ml): 3242, Standard deviation: 217, CV(%): 6.7

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Details zu CD25

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Andere Bezeichnung: IL2RA

Hintergrund:

Protein Function: Receptor for interleukin-2.

Background: CD25 is the alpha chain of the IL-2 receptor. It is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes that associates with CD122 to form a heterodimer that can act as a high-affinity receptor for IL-2. CD25 has been used as a marker to identify CD4+FoxP3+ regulatory T cells in mice. By in situ hybridization, the IL2R gene was mapped to 10p15-p14. Its soluble form, called sIL-2R may be elevated in these diseases and is occasionally used to track disease progression.

Synonyms: Interleukin-2 receptor subunit alpha,IL-2 receptor subunit alpha,IL-2-RA,IL-2R subunit alpha,IL2-RA,p55,CD25,Il2ra,Il2r,

Full Gene Name: Interleukin-2 receptor subunit alpha

Cellular Localisation: Membrane, Single-pass type I membrane protein.

Gen-ID: 16184

UniProt: P01590

Pathways: JAK-STAT Signalweg, Growth Factor Binding, Activated T Cell Proliferation