PLAT ELISA Kit

Produktnummer ABIN1672825

Human PLAT ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human PLAT und wurde in 1 Publikation erwähnt.

Kurzübersicht für PLAT ELISA Kit (ABIN1672825)

Target: PLAT (Plasminogen Activator, Tissue (PLAT))

Bindungsspezifität: AA 1-562

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.2-2000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate), Urine

Produktdetails PLAT ELISA Kit

Untere Nachweisgrenze: 31.2 pg/mL

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human PLAT/TPA

Marke: PicoKine™

Analytische Methode: Quantitative

Spezifität: Expression system for standard: CHO
Immunogen sequence: M1-P562

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <10pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: CHO
Immunogen sequence: M1-P562

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Kommentare:

Sequence similarities: Belongs to the peptidase S1 family.

Tissue Specificity: Synthesized in numerous tissues (including tumors) and secreted into most extracellular body fluids, such as plasma, uterine fluid, saliva, gingival crevicular fluid, tears, seminal fluid, and milk.

Plattentyp: Pre-coated

Protokoll: human PLAT ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for PLAT has been precoated onto 96-well plates. Standards(CHO, M1-P562) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PLAT is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human PLAT amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human PLAT standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma (heparin, EDTA, citrate) or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human PLAT standard solution and each sample be measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 191, Standard deviation: 11.65, CV(%): 6.1
• Sample 2: n=16, Mean(pg/ml): 512, Standard deviation: 24.1, CV(%): 4.7
• Sample 3: n=16, Mean(pg/ml): 925, Standard deviation: 50, CV(%): 5.4,
• Sample 1: n=24, Mean(pg/ml): 244, Standard deviation: 17.57, CV(%): 7.2
• Sample 2: n=24, Mean(pg/ml): 732, Standard deviation: 42.5, CV(%): 5.8
• Sample 3: n=24, Mean(pg/ml): 1216, Standard deviation: 81.5, CV(%): 6.7

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Referenzen für PLAT ELISA Kit (ABIN1672825)

Hu, Chang, Zhang, Wang, Lei, Li, Ren, Ji: "Mechanical Adaptability of the MMP-Responsive Film Improves the Functionality of Endothelial Cell Monolayer." in: Advanced healthcare materials, Vol. 6, Issue 14, (2018) (PubMed).

Details zu PLAT

Target: PLAT (Plasminogen Activator, Tissue (PLAT))

Andere Bezeichnung: PLAT

Hintergrund:

Protein Function: Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. Plays a direct role in facilitating neuronal migration.

Background: Plasminogen activator, tissue, also called PLAT or TPA is a serine protease. This gene encodes tissue-type plasminogen activator, a secreted serine protease which converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. Tissue-type plasminogen activator is synthesized as a single chain which is cleaved by plasmin to a two chain disulfide linked protein. This gene was mapped to 8p11.21. This enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding, decreased activity leads to hypofibrinolysis which can result in thrombosis or embolism.

Synonyms: Tissue-type plasminogen activator,t-PA,t-plasminogen activator,tPA,3.4.21.68,Alteplase,Reteplase,Tissue-type plasminogen activator chain A,Tissue-type plasminogen activator chain B,PLAT,

Full Gene Name: Tissue-type plasminogen activator

Cellular Localisation: Secreted, extracellular space.

Gen-ID: 5327

UniProt: P00750

Pathways: Autophagie, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling, SARS-CoV-2 Protein Interaktom