IL-21 ELISA Kit

Produktnummer ABIN1672800

Dieses Maus IL-21 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Maus IL-21 zu quantifizieren. Dieses ELISA Kit wurde in 3+ Publikationen zitiert.

Kurzübersicht für IL-21 ELISA Kit (ABIN1672800)

Target: IL-21 (IL21) (Interleukin 21 (IL21))

Bindungsspezifität: AA 25-146

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 62.5-4000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum

Produktdetails IL-21 ELISA Kit

Untere Nachweisgrenze: 62.5 pg/mL

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse IL-21

Marke: PicoKine™

Analytische Methode: Quantitative

Spezifität: Expression system for standard: E.coli
Immunogen sequence: P25-S146

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <10pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: E.coli
Immunogen sequence: P25-S146

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plattentyp: Pre-coated

Protokoll: mouse IL-21 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-21 has been precoated onto 96-well plates. Standards(E.coli, P25-S146) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-21 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-21 amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse IL-21 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse IL-21 standard solution and each sample be measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 316, Standard deviation: 15.8, CV(%): 5
• Sample 2: n=16, Mean(pg/ml): 2196, Standard deviation: 80.4, CV(%): 6.2
• Sample 3: n=16, Mean(pg/ml): 2113, Standard deviation: 99.31, CV(%): 4.7,
• Sample 1: n=24, Mean(pg/ml): 427, Standard deviation: 25.2, CV(%): 5.9
• Sample 2: n=24, Mean(pg/ml): 1450, Standard deviation: 97.15, CV(%): 6.7
• Sample 3: n=24, Mean(pg/ml): 2485, Standard deviation: 159, CV(%): 6.4

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Referenzen für IL-21 ELISA Kit (ABIN1672800)

Li, Jin, Wang, Li, Liu, Chang, Wang, Liu: "Live attenuated Salmonella displaying HIV-1 10E8 epitope on fimbriae: systemic and mucosal immune responses in BALB/c mice by mucosal administration." in: Scientific reports, Vol. 6, pp. 29556, (2018) (PubMed).

Shen, Yang, Yang, Wang, Cui, Zhou, Liu, Pan, Liu, Zhang, Zhang, Xie, Liu: "Hepatitis B virus persistence in mice reveals IL-21 and IL-33 as regulators of viral clearance." in: Nature communications, Vol. 8, Issue 1, pp. 2119, (2017) (PubMed).

Yu, Tian, Wang, Guo, Li, Wang, Li, Zhang, Zhuang, Ling: "An HIV-1 envelope immunogen with W427S mutation in CD4 binding site induced more T follicular helper memory cells and reduced non-specific antibody responses." in: PLoS ONE, Vol. 9, Issue 12, pp. e115047, (2014) (PubMed).

Details zu IL-21

Target: IL-21 (IL21) (Interleukin 21 (IL21))

Andere Bezeichnung: IL21

Hintergrund:

Background: Interleukin-21 is a protein that in humans is encoded by the IL21 gene. Interleukin 21 is a cytokine that has potent regulatory effects on cells of the immune system, including natural killer(NK) cells and cytotoxic T cells that can destroy virally infected or cancerous cells. By radiation hybrid analysis, the IL21 gene is mapped to chromosome 4q26-q27, near the IL2 gene. IL21 stimulates B-cell proliferation in an anti-CD40-dependent manner but inhibits proliferation stimulated by IL4 plus anti-IgM. The biologic effects of IL21 on NK cells differ between species, with human IL21 enhancing NK cell proliferation and murine IL21 inhibiting NK cell proliferation. IL21 is a cytokine highly expressed by mouse T(H)17 cells.

Synonyms: Interleukin ,Il21 ,mCG_12674 ,

Full Gene Name: Interleukin

Gen-ID: 60505

UniProt: Q5SUE2

Pathways: JAK-STAT Signalweg, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response