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HBEGF ELISA Kit

Dieses Human HBEGF ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human HBEGF zu quantifizieren.
Produktnummer ABIN1672797

Kurzübersicht für HBEGF ELISA Kit (ABIN1672797)

Target

Alle HBEGF ELISA Kits anzeigen
HBEGF (Heparin-Binding EGF-Like Growth Factor (HBEGF))

Bindungsspezifität

AA 63-148

Reaktivität

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Human

Nachweismethode

Colorimetric

Methodentyp

Sandwich ELISA

Detektionsbereich

15.6-1000 pg/mL

Applikation

ELISA

Proben

Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
  • Untere Nachweisgrenze

    15.6 pg/mL

    Verwendungszweck

    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human HBEGF

    Marke

    PicoKine™

    Analytische Methode

    Quantitative

    Spezifität

    Expression system for standard: sf21
    Immunogen sequence: D63-L148

    Kreuzreaktivität (Details)

    There is no detectable cross-reactivity with other relevant proteins.

    Sensitivität

    <10pg/mL

    Benötigtes Material

    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

    Immunogen

    Expression system for standard: sf21
    Immunogen sequence: D63-L148
  • Applikationshinweise

    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

    Kommentare

    Sequence similarities: Contains 1 EGF-like domain.

    Plattentyp

    Pre-coated

    Protokoll

    human HBEGF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for HBEGF has been precoated onto 96-well plates. Standards(sf21, D63-L148) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for HBEGF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human HBEGF amount of sample captured in plate.

    Testdurchführung

    Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human HBEGF standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human HBEGF standard solution and each sample be measured in duplicate.

    Testpräzision

    • Sample 1: n=16, Mean(pg/ml): 92.7, Standard deviation: 5.10, CV(%): 5.5
    • Sample 2: n=16, Mean(pg/ml): 369, Standard deviation: 16.97, CV(%): 4.6
    • Sample 3: n=16, Mean(pg/ml): 621, Standard deviation: 23.6, CV(%): 3.8,
    • Sample 1: n=24, Mean(pg/ml): 102, Standard deviation: 8.26, CV(%): 8.1
    • Sample 2: n=24, Mean(pg/ml): 376, Standard deviation: 27.82, CV(%): 7.4
    • Sample 3: n=24, Mean(pg/ml): 618, Standard deviation: 42.02, CV(%): 6.8

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Handhabung

    Avoid multiple freeze-thaw cycles.

    Lagerung

    -20 °C,4 °C

    Informationen zur Lagerung

    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

    Haltbarkeit

    12 months
  • Target Alle HBEGF ELISA Kits anzeigen

    HBEGF (Heparin-Binding EGF-Like Growth Factor (HBEGF))

    Andere Bezeichnung

    HBEGF

    Hintergrund

    Protein Function: Growth factor that mediates its effects via EGFR, ERBB2 and ERBB4. Required for normal cardiac valve formation and normal heart function. Promotes smooth muscle cell proliferation. May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts, but not endothelial cells. It is able to bind EGF receptor/EGFR with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor.

    Background: HBEGF(Heparin-binding EGF-like growth factor), also known as HEGFL or DTR, is a member of the EGF family of proteins that in humans is encoded by the HBEGF gene. The HBEGF gene is assigned to chromosome 5, thus confirming the assignment of the gene on the basis of its role in relation to diphtheria toxin susceptibility. HB-EGF is an 87 amino acid glycoprotein which displays highly regulated gene expression. It has been shown to play a role in wound healing, cardiac hypertrophy and heart development and function. HB-EGF binding and activation of EGF receptors plays a critical role during cardiac valve tissue development and the maintenance of normal heart function in adults.

    Synonyms: Proheparin-binding EGF-like growth factor,Heparin-binding EGF-like growth factor,HB-EGF,HBEGF,Diphtheria toxin receptor,DT-R,HBEGF,DTR, DTS, HEGFL,

    Full Gene Name: Proheparin-binding EGF-like growth factor

    Cellular Localisation: Heparin-binding EGF-like growth factor: Secreted, extracellular space. Mature HB-EGF is released into the extracellular space and probably binds to a receptor.

    Gen-ID

    1839

    UniProt

    Q99075

    Pathways

    RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung
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