TNFRSF18 ELISA Kit (Tumor Necrosis Factor Receptor Superfamily, Member 18)

Details for Product TNFRSF18 ELISA Kit No. ABIN1672796, Anbieter: Anmelden zum Anzeigen
Antigen
  • GITR
  • TNFRSF18
  • AITR
  • Gitr
  • CD357
  • GITR-D
  • TNF receptor superfamily member 18
  • tumor necrosis factor receptor superfamily, member 18
  • TNFRSF18
  • Tnfrsf18
Epitop
AA 22-153
Reaktivität
Maus
Alternativen
Kits mit alternativen Reaktivitäten:
14
6
Methodentyp
Sandwich ELISA
Detektionsbereich
62.5-4000 pg/mL
Untere Nachweisgrenze
62.5 pg/mL
Applikation
ELISA
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Verwendungszweck Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse TNFRSF18/GITR
Marke PicoKine™
Proben Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
Analytische Methode Quantitative
Nachweismethode Colorimetric
Immunogen Expression system for standard: NSO
Immunogen sequence: S22-H153
Spezifität Expression system for standard: NSO
Immunogen sequence: S22-H153
Kreuzreaktivität (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivität <10pg/mL
Benötigtes Material Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Plasmids, Primers & others Plasmide, Primers & weitere TNFRSF18 products on genomics-online (e.g. as negative or positive controls)
Antigen
Andere Bezeichnung TNFRSF18 (TNFRSF18 ELISA Kit Abstract)
Hintergrund

Protein Function: Receptor for TNFSF18. Seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. Mediated NF-kappa-B activation via the TRAF2/NIK pathway (By similarity). .

Background: Tumor necrosis factor receptor superfamily member 18(TNFRSF18), also called GITR or AITR is a protein that in humans is encoded by the TNFRSF18 gene. This gene is mapped to 1p36.33. This gene encodes a member of the TNF-receptor superfamily. The encoded receptor has been shown to have increased expression upon T-cell activation, and it is thought to play a key role in dominant immunological self-tolerance maintained by CD25(+)CD4(+) regulatory T cells. Knockout studies in mice also suggest the role of this receptor is in the regulation of CD3-driven T-cell activation and programmed cell death. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

Synonyms: Tumor necrosis factor receptor superfamily member 18,Glucocorticoid-induced TNFR-related protein,CD357,Tnfrsf18,Gitr,

Full Gene Name: Tumor necrosis factor receptor superfamily member 18

Cellular Localisation: Isoform A: Cell membrane, Single-pass type I membrane protein.
Gen-ID 21936
UniProt O35714
Applikations-hinweise Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Kommentare

Tissue Specificity: Preferentially expressed in activated T lymphocytes.

Plattentyp Pre-coated
Protokoll mouse TNFRSF18 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TNFRSF18 has been precoated onto 96-well plates. Standards(NSO, S22-H153) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFRSF18 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse TNFRSF18 amount of sample captured in plate.
Testdurchführung

Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse TNFRSF18 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse TNFRSF18 standard solution and each sample be measured in duplicate.

Testpräzision
  • Sample 1: n=16, Mean(pg/ml): 228, Standard deviation: 10.5, CV(%): 4.6
  • Sample 2: n=16, Mean(pg/ml): 1805, Standard deviation: 97.47, CV(%): 5.4
  • Sample 3: n=16, Mean(pg/ml): 3208, Standard deviation: 96.24, CV(%): 3,
  • Sample 1: n=24, Mean(pg/ml): 324, Standard deviation: 17.82, CV(%): 5.5
  • Sample 2: n=24, Mean(pg/ml): 2125, Standard deviation: 144.5, CV(%): 6.8
  • Sample 3: n=24, Mean(pg/ml): 3154, Standard deviation: 220.8, CV(%): 7
Beschränkungen Nur für Forschungszwecke einsetzbar
Handhabung Avoid multiple freeze-thaw cycles.
Lagerung -20 °C,4 °C
Informationen zur Lagerung Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Haltbarkeit 12 months
Bilder des Herstellers
ELISA image for Tumor Necrosis Factor Receptor Superfamily, Member 18 (TNFRSF18) ELISA Kit (ABIN1672796) Mouse TNFRSF18/GITR PicoKine ELISA Kit standard curve
Allgemeine Veröffentlichungen Grohmann, Volpi, Fallarino, Bozza, Bianchi, Vacca, Orabona, Belladonna, Ayroldi, Nocentini, Boon, Bistoni, Fioretti, Romani, Riccardi, Puccetti: "Reverse signaling through GITR ligand enables dexamethasone to activate IDO in allergy." in: Nature medicine, Vol. 13, Issue 5, pp. 579-86, 2007 (PubMed).

Ji, Liao, Faubion, Abadía-Molina, Cozzo, Laroux, Caton, Terhorst: "Cutting edge: the natural ligand for glucocorticoid-induced TNF receptor-related protein abrogates regulatory T cell suppression." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 172, Issue 10, pp. 5823-7, 2004 (PubMed).

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