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GM-CSF ELISA Kit (Colony Stimulating Factor 2 (Granulocyte-Macrophage)) ELISA Kit

CSF2 Reaktivität: Human AA 18-144 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Produktnummer ABIN1672729
$400.00
Zzgl. Versandkosten $45.00
96 tests
Lieferung in 4 bis 6 Werktagen
  • Target Alle GM-CSF (CSF2) ELISA Kits anzeigen
    GM-CSF (CSF2)
    Bindungsspezifität
    AA 18-144
    Reaktivität
    • 13
    • 8
    • 6
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    15.6-1000 pg/mL
    Untere Nachweisgrenze
    15.6 pg/mL
    Applikation
    ELISA
    Verwendungszweck
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human GM-CSF
    Marke
    PicoKine™
    Proben
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)
    Analytische Methode
    Quantitative
    Spezifität
    Expression system for standard: E.coli
    Immunogen sequence: A18-E144
    Kreuzreaktivität (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivität
    <1pg/mL
    Benötigtes Material
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: E.coli
    Immunogen sequence: A18-E144
    Featured
    Zu unserem meistverkauften CSF2 ELISA Kit
  • Applikationshinweise
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Kommentare

    Sequence similarities: Belongs to the GM-CSF family.

    Plattentyp
    Pre-coated
    Protokoll
    human GM-CSF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for GM-CSF has been precoated onto 96-well plates. Standards(E.coli, A18-E144) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for GM-CSF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human GM-CSF amount of sample captured in plate.
    Testdurchführung

    Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human GM-CSF standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human GM-CSF standard solution and each sample be measured in duplicate.

    Testpräzision
    • Sample 1: n=16, Mean(pg/ml): 104, Standard deviation: 4.58, CV(%): 4.4
    • Sample 2: n=16, Mean(pg/ml): 485, Standard deviation: 15.52, CV(%): 3.2
    • Sample 3: n=16, Mean(pg/ml): 621, Standard deviation: 27.95, CV(%): 4.5,
    • Sample 1: n=24, Mean(pg/ml): 107, Standard deviation: 6.53, CV(%): 6.1
    • Sample 2: n=24, Mean(pg/ml): 463, Standard deviation: 29.63, CV(%): 6.4
    • Sample 3: n=24, Mean(pg/ml): 618, Standard deviation: 35.23, CV(%): 5.7
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    Avoid multiple freeze-thaw cycles.
    Lagerung
    -20 °C,4 °C
    Informationen zur Lagerung
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Haltbarkeit
    12 months
  • Target Alle GM-CSF (CSF2) ELISA Kits anzeigen
    GM-CSF (CSF2)
    Andere Bezeichnung
    CSF2 (CSF2 Produkte)
    Synonyme
    GMCSF, GM-CSF, CSF2, Csfgm, Gm-CSf, MGI-IGM, Gm-csf, Gmcsf, CSF, colony stimulating factor 2, colony stimulating factor 2 (granulocyte-macrophage), CSF2, Csf2
    Hintergrund

    Protein Function: Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.

    Background: Granulocyte-macrophage colony-stimulating factor(GM-CSF) is also symbolized CSF2. Human GM-CSF is a glycoprotein that is essential for the in vitro proliferation and differentiation of precursor cells into mature granulocytes and macrophages. The human cDNA clones contain a single open-reading frame encoding a protein of 144 amino acids with a predicted molecular mass of 16,293 daltons and show 69 % nucleotide homology and 54 % amino acid homology to mouse GM-CSF. The gene for human GM-CSF appears to exist as a single-copy gene. Human GM-CSF is a 22,000-dalton glycoprotein that stimulates the growth of myeloid progenitor cells and acts directly on mature neutrophils. The GM-CSF gene is localized by somatic cell hybrid analysis and in situ hybridization to human chromosome region 5q21-5q32, which is involved in interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. A complementary DNA for the T lymphocyte-derived lymphokine, GM-CSF has been cloned, and recombinant GM-CSF protein has been expressed in yeast and purified to homogeneity. This purified human recombinant GM-CSF stimulates peripheral blood monocytes in vitro to become cytotoxic for the malignant melanoma cell line A375.

    Synonyms: Granulocyte-macrophage colony-stimulating factor,GM-CSF,Colony-stimulating factor,CSF,Molgramostin,Sargramostim,CSF2,GMCSF,

    Full Gene Name: Granulocyte-macrophage colony-stimulating factor

    Cellular Localisation: Secreted.
    Gen-ID
    1437
    UniProt
    P04141
    Pathways
    JAK-STAT Signalweg, Cellular Response to Molecule of Bacterial Origin
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