Immunoglobulin isotyping

Produktnummer ABIN1113186

Kurzübersicht für Immunoglobulin isotyping (ABIN1113186)

Target: Ig

Reaktivität: Maus

Nachweismethode: Colorimetric

Applikation: Isotyping (IsoT)

Proben: Cell Culture Supernatant, Purified Protein

Produktdetails

Bindungsspezifität: Heavy & Light Chain

Konjugat: HRP

Verwendungszweck: The method employs the quantitative sandwich enzyme immunoassay technique.

Marke: FastELISA®

Spezifität: Mouse IgG1, IgG2a, IgG2b, IgG3, IgM, Kappa chain,

Produktmerkmale: The mouse immunoglobulin isotyping kit provides a rapid and easy method (one step ELISA) to characterize mouse monoclonal antibody isotypes in cell culture supernatants or purified antibodies preparations.

Anwendungsinformationen

Kommentare:

One Step ELISA 30 min total time

Testdauer: < 1 h

Plattentyp: Pre-coated

Protokoll: Anti-mouse antibodies specific to each of the common light and heavy chains are pre-coated in the wells. Samples are pipetted into microwells and Ig present in the sample are bound by the capture antibody. Then, a HRP (horseradish peroxidase) conjugated anti-mouse IgG (H+L) antibody is pipetted and incubated simultaneously with samples. After washing microwells in order to remove any non specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops if the specific immunoglobulin is present in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.

Aufbereitung der Reagenzien:

dilution

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Antigendetails

Target: Ig

Andere Bezeichnung: Immunoglobulins

Hintergrund: Isotyping of mouse immunoglobulins