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Immunoglobulin isotyping

IsoT Reaktivität: Maus Colorimetric Cell Culture Supernatant, Purified Protein Pre-coated
Produktnummer ABIN1113187
  • Target Alle Ig Kits anzeigen
    Ig
    Bindungsspezifität
    Heavy & Light Chain
    Reaktivität
    • 4
    • 4
    • 1
    • 1
    Maus
    Nachweismethode
    Colorimetric
    Konjugat
    HRP
    Applikation
    Isotyping (IsoT)
    Verwendungszweck
    The method employs the quantitative sandwich enzyme immunoassay technique.
    Marke
    FastELISA®
    Proben
    Cell Culture Supernatant, Purified Protein
    Spezifität
    Mouse IgG1, IgG2a, IgG2b, IgG3, IgM, Kappa chain,
    Produktmerkmale
    The mouse immunoglobulin isotyping kit provides a rapid and easy method (one step ELISA) to characterize mouse monoclonal antibody isotypes in cell culture supernatants or purified antibodies preparations.
    Top Product
    Discover our top product Ig ELISA Kit
  • Kommentare

    One Step ELISA 30 min total time

    Testdauer
    < 1 h
    Plattentyp
    Pre-coated
    Protokoll
    Anti-mouse antibodies specific to each of the common light and heavy chains are pre-coated in the wells. Samples are pipetted into microwells and Ig present in the sample are bound by the capture antibody. Then, a HRP (horseradish peroxidase) conjugated anti-mouse IgG (H+L) antibody is pipetted and incubated simultaneously with samples. After washing microwells in order to remove any non specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops if the specific immunoglobulin is present in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
    Aufbereitung der Reagenzien

    dilution

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    4 °C
    Haltbarkeit
    12 months
  • Target
    Ig
    Andere Bezeichnung
    Immunoglobulins (Ig Produkte)
    Synonyme
    ATPIG Kit, ATPIQ Kit, A330005H02Rik Kit, AI315324 Kit, Ig Kit, ATPase phospholipid transporting 11C Kit, ATPase, class VI, type 11C Kit, ATP11C Kit, Atp11c Kit
    Hintergrund
    Isotyping of mouse immunoglobulins
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