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IL-7 ELISA Kit

Human IL-7 ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human IL-7 in Cell Culture Cells, Plasma und Serum
Produktnummer ABIN1112656

Kurzübersicht für IL-7 ELISA Kit (ABIN1112656)

Target

Alle IL-7 (IL7) ELISA Kits anzeigen
IL-7 (IL7) (Interleukin 7 (IL7))

Reaktivität

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Human

Nachweismethode

Colorimetric

Methodentyp

Sandwich ELISA

Detektionsbereich

15.6-1000 pg/mL

Applikation

ELISA

Proben

Cell Culture Cells, Plasma, Serum
  • Untere Nachweisgrenze

    15.6 pg/mL

    Verwendungszweck

    For quantitative detection of IL-7 in Human serum, plasma or cell.

    Analytische Methode

    Quantitative

    Sensitivität

    < 1 pg/mL

    Bestandteile

    1. One 96-well plate pre-coated with anti-Human IL-7 antibody 2. Lyophilized Human IL-7 standards: 2 tubes (10ng / tube) 3. Sample / Standard diluent buffer: 30ml 4. Biotin conjugated anti-Human IL-7 antibody (Concentrated): 130 µl.

    Benötigtes Material

    1. 37 °C incubator 2. Microplate reader (wavelength: 450nm) 3. Precise pipette and disposable pipette tips 4. Automated plate washer 5. ELISA shaker 6. 1.5ml of Eppendorf tubes 7. Plate cover 8. Absorbent filter papers 9. Plastic or glass container with volume of above 1L
  • Kommentare

    This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-IL-7 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IL-7 polyclonal antibody was used as detection antibodies. The standards test samples and biotin conjugated detection antibody were added - the wells subsequently and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used - visualize HRP enzymatic reaction. TMB was catalyzed by HRP - produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional - the IL-7 amount of sample captured in plate. Read the O.D. absorbance at 450 nm in a microplate reader and then the concentration of IL-7 can be calculated.

    Plattentyp

    Pre-coated

    Aufbereitung der Reagenzien

    1. Before the experiment, centrifuge each kit component for several minutes to bring down all reagents to the bottom of tubes. 2. It is recommend to measure each standard and sample in duplicate. 3. Do NOT let the plate completely dry at any time! Since the dry condition can inactivate the biological material on the plate. 4. Do not reuse pipette tips and tubes to avoid cross contamination. 5. Do not use the expired components and the components from different batches. 6. To avoid the marginal effect of plate incubation for temperature differences (the marginal wells always get stronger reaction), it is recommend to equilibrate the ABC working solution and TMB substrate for at least 30 min at room temperature (37°C ) before adding to wells.The TMB substrate (Kit Component 8) is colorless and transparent before use, if not, please contact us for replacement.

    Aufbereitung der Proben

    Preparation of sample and reagents 1. Sample Isolate the test samples soon after collecting, then, analyze immediately (within 2 hours). Or aliquot and store at -20 °C for long term. Avoid multiple freeze-thaw cycles.
    Cell: Lyse cells thoroughly, centrifuge at 10000-14000 X g for 3-5 min, collect supernatant, analyze immediately or aliquot and store at -20 °C.
    Serum: Coagulate the serum at room temperature (about 30 min). Centrifuge at approximately 2000 × g for 20 min. Analyze the serum immediately or aliquot and store at -20 °C .
    Plasma: Collect plasma with citrate, heparin or EDTA as the anticoagulant. Centrifuge for 15 min at 1000 x g within 30 min of collection. Analyze immediately or aliquot and store frozen at -20 °C. Note: 1. Coagulate blood samples completely, then, centrifuge, and avoid hemolysis and particle. 2. NaN3 can not be used as test sample preservative, since it is the inhibitor for HRP.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Konservierungsmittel

    Sodium azide, Thimerosal (Merthiolate)
  • Target Alle IL-7 (IL7) ELISA Kits anzeigen

    IL-7 (IL7) (Interleukin 7 (IL7))

    Andere Bezeichnung

    IL-7

    Hintergrund

    Interleukin 7(IL-7) is a hematopoietic growth factor secreted by stromal cells in the red marrow and thymus. It is also produced by keratinocytes, dendritic cells, hepatocytes, neurons, and epithelial cells. IL-7 is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. This cytokine is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRß) during early T cell development. Il-7 promotes hematological malignacies (acute lymphoblastic leukemia, T cell lymphoma), and the elevated levels of IL-7 have also been detected in the plasma of HIV-infected patients.

    Pathways

    JAK-STAT Signalweg
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