NF-kappa B (NF-kB) Activation Assay Kit

Produktnummer ABIN1110858

Kurzübersicht für NF-kappa B (NF-kB) Activation Assay Kit (ABIN1110858)

Target: NFkB (Nuclear Factor kappa B (NFkB))

Reaktivität: Human

Applikation: Activity Assay (AcA), Western Blotting (WB)

Produktdetails

Verwendungszweck: Rapid assay to quantitate NF-kappa B activation using cell fractionation into nuclear and cytoplasmic components

Spezifität: This NF-KB activation assay follows the translocation of the p65 (RelA) subunit of NF-kB into the nucleus, a hallmark of NF-kappa B activation. Cytoplasmic and nuclear fractions are isolated, and Western blots of the fractions are used to quantify the relative p65 concentration. The ratio of nuclear relative to cytoplasmic p65 concentration provides a numerical estimate of NF-kappa B activation.

Produktmerkmale: This NF-KB Activation Assay Kit provides all the reagents required to fractionate cells into cytoplasmic and nuclear fractions, as well as a selective and high affinity antibody to detect p65 in Western blots.

Bestandteile:

• Cytoplasmic Fractionation Reagent (CER-1, 55mL)
• Nuclear Fractionation Reagent (NER-1, 3.5 mL)
• DTT (sufficient to make a 1M solution, volume 64 microliters)
• Rabbit Polyclonal IgG Antibody to p65. (100 µL Use at 1:100 - 1:400 dilution for Western blots)

Anwendungsinformationen

Applikationshinweise: Use at 1:400 dilution for Western blots

Kommentare:

Cytoplasmic Fractionation Reagent (55 mL)
Nuclear Fractionation Reagent (3.5 mL)
DTT (sufficient to make a 1 M solution, volume 64 µL)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: p65Ab: Aliquot, -20°C.
HRPAb: 4°C.
Other components: -20°C

Referenzen

Shi, Luo, Wang, Long: "Incorporation of β-sitosterol into the membrane prevents tumor necrosis factor-α-induced nuclear factor-κB activation and gonadotropin-releasing hormone decline." in: Steroids, Vol. 96, pp. 1-6, (2015) (PubMed).

Dattaroy, Pourhoseini, Das, Alhasson, Seth, Nagarkatti, Michelotti, Diehl, Chatterjee: "Micro-RNA 21 inhibition of SMAD7 enhances fibrogenesis via leptin-mediated NADPH oxidase in experimental and human nonalcoholic steatohepatitis." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 308, Issue 4, pp. G298-312, (2015) (PubMed).

Zou, Ding, Zhan, Liu, Pan, Xia: "Glyoxalase I is differentially expressed in cutaneous neoplasms and contributes to the progression of squamous cell carcinoma." in: The Journal of investigative dermatology, Vol. 135, Issue 2, pp. 589-98, (2015) (PubMed).

Martinotti, Ranzato, Parodi, Vitale, Burlando: "Combination of ascorbate/epigallocatechin-3-gallate/gemcitabine synergistically induces cell cycle deregulation and apoptosis in mesothelioma cells." in: Toxicology and applied pharmacology, Vol. 274, Issue 1, pp. 35-41, (2013) (PubMed).

Antigendetails

Target: NFkB (Nuclear Factor kappa B (NFkB))

Andere Bezeichnung: NF-kappa B