Ascorbic Acid Assay Kit

Produktnummer ABIN1000291

Kurzübersicht für Ascorbic Acid Assay Kit (ABIN1000291)

Target: Vitamin C (Ascorbic Acid (Vitamin C))

Applikation: Biochemical Assay (BCA)

Proben: Cell Culture Supernatant, Cell Culture Supernatant, Milk, Plasma, Saliva, Serum, Urine

Produktdetails

Produktmerkmale: Use 20 µL samples. Linear detection range: colorimetric assay 6 to 1000 µM, fluorimetric assay 1 to 100 µM ascorbic acid.

Bestandteile: Assay Buffer: 10 mL. Enzyme Mix: 120 µL. Dye Reagent: 120 µL. Standard: 400 µL 10 mM ascorbic acid.

Benötigtes Material: Pipetting devices, centrifuge tubes, clear flat-bottom uncoated 96-well plates, optical density plate reader, black flat-bottom uncoated 96-well plates, fluorescence plate reader.

Anwendungsinformationen

Applikationshinweise: Assays: ascorbic acid in biological samples such as serum, plasma, urine, saliva, milk, tissue, and cell culture.
Drug Discovery/Pharmacology: effects of drugs on ascorbic acid metabolism.

Ergebnisberechnung:

Subtract blank value (#4) from the standard values and plot the OD or F against standard concentrations.
Conversions: 1 mM ascorbic acid equals 17.6 mg/dL, 0.0176% or 176 ppm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Antigendetails

Target: Vitamin C (Ascorbic Acid (Vitamin C))

Andere Bezeichnung: Ascorbic Acid

Substanzklasse: Chemical

Hintergrund: Quantitative determination of ascorbic acid by colorimetric (570nm) or fluorimetric (530nm/590nm) methods.
Procedure: 10 min.

Ascorbic acid (the L-enantiomer commonly known as vitamin C) is an important antioxidant found in living organisms and applied as additives in food and other industrial processes. By reacting with reactive oxygen species, it protects the cell from oxidative damages. This method provides a simple, direct and high-throughput assay for measuring ascorbic acid. In this assay, ascorbic acid is oxidized by ascorbate oxidase resulting in the production of H2O2 which reacts with a specific dye to form a pink colored product. The color intensity at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the ascorbic acid concentration in the sample.