GST Antikörper
(Glutathione S Transferase (GST))
The GST tag is widely used in recombinant protein expression and purification. GST antibodies used to detect GST tags (e.g. in Western Blot) serve several purposes:
- Protein Detection: Antibodies against GST tag are used to detect the presence of GST Fusion proteins in various samples, including cell lysates and purified protein preparations. This is commonly done through techniques like Western blotting (WB), enzyme-linked immunosorbent assay (ELISA), and immunohistochemistry (IHC) - with Western Blot being the predominant method.
- Protein Purification: GST is often used as an affinity tag for protein purification. An antibody against GST can be used to verify the successful purification of a GST fusion protein by detecting its presence in eluted fractions.
- Immunoprecipitation: Antibodies against GST can be used to immunoprecipitate GST Fusion proteins from complex mixtures, allowing researchers to isolate and study specific protein interactions and complexes.
- Cellular Localization: Immunofluorescence techniques (IF) can use anti-GST antibodies to determine the subcellular localization of GST-tagged proteins within cells.
- Enzyme Activity Assays: GST activity can be measured using enzyme activity assays, and anti-GST antibodies can be employed in these assays to detect the activity of GST-fused proteins.
- Protein-Protein Interaction Studies: Anti-GST antibodies can be used in co-immunoprecipitation (Co-IP) experiments to study protein-protein interactions involving GST-fused proteins.
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GST
Reaktivität: Chemical
WB, ELISA
Wirt: Maus
Monoclonal
vpg66
Biotin
1 image
1 reference
GST
Reaktivität: Schistosoma japonicum
WB, ELISA, IP
Wirt: Maus
Monoclonal
2F10B9
unconjugated
Rockland 600-101-200
GST
Reaktivität: Schistosoma japonicum
WB, ELISA, IP, IHC
Wirt: Ziege
Polyclonal
unconjugated
7 Referenzen
Rockland Original
GST
Reaktivität: Tag
WB, ELISA, IP, IHC, FACS, ICC
Wirt: Maus
Monoclonal
6G9C6
unconjugated
1 image
GST
Reaktivität: Nicotiana tabacum
WB
Wirt: Kaninchen
Polyclonal
unconjugated
1 image
GST
Reaktivität: Schistosoma japonicum
WB, ELISA, IP, DB
Wirt: Maus
Monoclonal
GST-B6
unconjugated
1 image
GST
Reaktivität: Schistosoma japonicum
WB, ELISA
Wirt: Maus
Monoclonal
3E2
unconjugated
1 image
GST
Reaktivität: Schistosoma japonicum
WB, ELISA
Wirt: Maus
Monoclonal
3E2
HRP
1 image
GST
Reaktivität: Schistosoma japonicum
WB, ELISA
Wirt: Maus
Monoclonal
3E2
FITC
1 image
GST
Reaktivität: Schistosoma japonicum
WB, ELISA
Wirt: Maus
Monoclonal
3E2
Biotin
1 image
GST
Reaktivität: Schistosoma japonicum
WB, ELISA
Wirt: Maus
Monoclonal
3E2
PE
1 image
GST
Reaktivität: Schistosoma japonicum
WB, ELISA
Wirt: Maus
Monoclonal
3E2
PerCP
1 image
GST
Reaktivität: Schistosoma japonicum
WB, ELISA
Wirt: Maus
Monoclonal
3E2
APC
1 image
GST
Reaktivität: Schistosoma japonicum
WB, ELISA
Wirt: Maus
Monoclonal
3E2
Atto 488
1 image
GST
Reaktivität: Schistosoma japonicum
WB, ELISA
Wirt: Maus
Monoclonal
3E2
Atto 594
1 image
GST
Reaktivität: Schistosoma japonicum
WB, ELISA
Wirt: Maus
Monoclonal
3E2
Atto 390
1 image
Aktuelle Publikationen für unsere GST Antikörper
: "Mus81-Mms4 endonuclease is an Esc2-STUbL-Cullin8 mitotic substrate impacting on genome integrity." in: Nature communications, Vol. 11, Issue 1, pp. 5746, (2020) (PubMed).: "NHDL, a recombinant VL/VH hybrid antibody control for IgG2/4 antibodies." in: mAbs, Vol. 12, Issue 1, pp. 1686319, (2020) (PubMed).
: "Vagus Nerve Stimulation Alleviates Hepatic Ischemia and Reperfusion Injury by Regulating Glutathione Production and Transformation." in: Oxidative medicine and cellular longevity, Vol. 2020, pp. 1079129, (2020) (PubMed).
: "Inosine induces context-dependent recoding and translational stalling." in: Nucleic acids research, Vol. 47, Issue 1, pp. 3-14, (2019) (PubMed).
: "Rosette Assay: Highly Customizable Dot-Blot for SH2 Domain Screening." in: Methods in molecular biology (Clifton, N.J.), Vol. 1555, pp. 437-451, (2018) (PubMed).
: "Brain Region and Isoform-Specific Phosphorylation Alters Kalirin SH2 Domain Interaction Sites and Calpain Sensitivity." in: ACS chemical neuroscience, Vol. 8, Issue 7, pp. 1554-1569, (2018) (PubMed).
: "ATP synthase F1 subunits recruited to centromeres by CENP-A are required for male meiosis." in: Nature communications, Vol. 9, Issue 1, pp. 2702, (2018) (PubMed).
: "InsP3R-SEC5 interaction on phagosomes modulates innate immunity to Candida albicans by promoting cytosolic Ca2+ elevation and TBK1 activity." in: BMC biology, Vol. 16, Issue 1, pp. 46, (2018) (PubMed).
: "Protective effect of dioscin against doxorubicin-induced cardiotoxicity via adjusting microRNA-140-5p-mediated myocardial oxidative stress." in: Redox biology, Vol. 16, pp. 189-198, (2018) (PubMed).
: "Local detection of PtdIns3P at autophagosome biogenesis membrane platforms." in: Autophagy, Vol. 13, Issue 9, pp. 1602-1612, (2018) (PubMed).
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