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FACS Antikörper

Fluoreszenz-aktivierte Zellsortierung (FACS) ist eine spezielle Art der Durchflusszytometrie. Sie sortiert ein heterogenes Gemisch biologischer Zellen in zwei oder mehr Behälter, jeweils eine Zelle nach der anderen, auf der Grundlage der spezifischen Lichtstreuungs- und Fluoreszenzeigenschaften jeder Zelle und der an sie gebundenen Antikörper.

Wichtige Targets

Arginase 1 Antibodies

CASP8 Antibodies

CCR2 Antibodies

CD14 Antibodies

FOXP3 Antibodies

IL17 Antibodies

Lamin B1 Antibodies

Alle Flow Cytometry Antibodies

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TUBB3 Antikörper (N-Term) (FITC)

TUBB3 Reaktivität: Human, Maus, Ratte, Hund, Schwein FACS, ICC Wirt: Maus Monoclonal TU-20 FITC

Immunofluorescence staining (mouse neuroblastoma cells) Immunofluorescence staining of Neuro2a mouse neuroblastoma cell line using anti-betaIII-tubulin (TU-20 ; green; 3 μg/ml).

Separation of MCF-7 cells stained using anti-human betaIII-Tubulin (TU-20) FITC antibody (10 μL reagent per million cells in 100 μL of cell suspension, red-filled) from MCF-7 cells stained using mouse IgG1 isotype control (MOPC-21) FITC antibody (concentration in sample 15 μg/mL, same as betaIII-Tubulin FITC concentration, black-dashed) in flow cytometry analysis (intracellular staining) of MCF-7 cell suspension.

Immunofluorescence staining of P-19 mouse embryonal carcinoma cell line stimulated to neuronal differentiation by retinoic acid.

Immunofluorescence staining (mouse embryonal carcinoma cells) Immunofluorescence staining of P-19 mouse embryonal carcinoma cell line stimulated to neuronal differentiation by retinoic acid.

Immunofluorescence staining of Neuro2a mouse neuroblastoma cell line using anti-betaIII-tubulin (TU-20 FITC, green, 1 μg/ml).

Immunofluorescence staining of Neuro2a mouse neuroblastoma cell line using anti-betaIII-tubulin (TU-20 FITC, green, 1 μg/mL).

6 Abbildungen

10 Referenzen

Produktnummer ABIN93912

Datenblatt (PDF)

beta Actin Antikörper

ACTB Reaktivität: Human, Maus, Ratte WB, IF, IHC (p), FACS, DNA Mic Wirt: Maus Monoclonal 137CT26-1-1 unconjugated

All lanes : Anti-ACTB Antibody at 1:2000 dilution + C6 cell lysate Lysates/proteins at 20 μg per lane.

Immunohistochemical analysis of paraffin-embedded H.

Confocal immunofluorescent analysis of ACTB Antibody (ABIN658990 and ABIN2838035) with Hela cell followed by Alexa Fluor® 488-conjugated goat anti-mouse lgG (green).

All lanes : Anti-ACTB Antibody at 1:1000 dilution Lane 1: A431 whole cell lysate Lane 2: C2C12 whole cell lysate Lane 3: C6 whole cell lysate Lane 4: Hela whole cell lysate Lane 5: MCF-7 whole cell lysate Lysates/proteins at 20 μg per lane.

Western blot analysis of anti-ACTB Antibody (ABIN658990 and ABIN2838035) in K562, HL-60,Hela cell line, mouse spleen, mouse liver tissue lysates, mouse NIH-3T3 cell line lysate and mouse cerebellum, mouse brain tissue lysates (35 μg/lane).

All lanes : Anti-ACTB Antibody at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: NIH-3T3 whole cell lysate Lysates/proteins at 20 μg per lane.

(ABIN658990 and ABIN2838035) staining ACTB in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

7 Abbildungen

12 Referenzen

Produktnummer ABIN658990

Datenblatt (PDF)

FcRn Antikörper

FcRn Reaktivität: Human ELISA, IF, FACS, cELISA, Func Wirt: Maus Monoclonal ADM31 unconjugated

Immunofluorescence: huSSECTM cell line overexpressing hFcRn-EGFP using clone ADM31 at 15µg/ml and Texas Red™ conjugated anti-mouse secondary.

Antibody- or peptide-mediated disruption of the human FcRn–albumin interactions decreases chemical hepatotoxicity.

293hFcRn-GFP cells were incubated with a range of concentrations of DVN1, ADM11, DVN21, DVN22, DVN23, DVN24, ADM31 or ADM32 at pH 6, then stained for functional binding with (A) 20 μg/mL hIgGAF647, or (B) 200 μg/mL HSAbiotin followed by streptavidin-PE.

Tracer plasma concentrations were determined by ELISA and plotted either as percent remaining as compared with the first time point plasma concentrations or as plasma concentrations ±SD.

ELISA binding of human, macaque, pig, dog, mouse, and rat FcRn toward ADM31 at pH 7.

Identification of monoclonal Abs that block albumin binding to hFcRn.

Immunofluorescence staining of huSSECTM cell line overexpressing hFcRn-EGFP using clone ADM31 at 15µg/ml and Texas Red™ conjugated anti-mouse secondary.

Flowcytometry of huSSECTM cell line overexpressing hFcRn-EGFP labeled with clone ADM31 primary Ab and Texas Red™ conjugated anti-mouse secondary.

8 Abbildungen

15 Referenzen

Produktnummer ABIN1774762

Datenblatt (PDF)

Liver Arginase Antikörper

KO Validated ARG1 Reaktivität: Human WB, ELISA, FACS, IHC (p), IF, IP, ICC, IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

WB Image Sample (20 ug of whole cell lysate) A: mouse liver 10% SDS PAGE antibody diluted at 1:50000

ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using arginase I , antibody at 1:200 dilution.

IHC-P Image Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using arginase I , antibody at 1:500 dilution.

WB Image Arginase 1 antibody detects Arginase 1 protein by western blot analysis.

Elevated right ventricular pressure, increased right ventricular weight and altered expression of Ddah1 and Arg1 in cavin-/- mice.

Elevated arginase1 expression and increased arginase inhibitor-induced relaxation of Cavin-1-/- arteries.

Elevated arginase1 expression and increased arginase inhibitor-induced relaxation of Cavin-1-/- arteries.

Arg-1 expression in neutrophils and lymph-node microenvironmentNeutrophils isolated from control and HL patients were analysed for Arg-1 expression (A), activity by measuring urea (B) and protein expression by Western Blot (C).

8 Abbildungen

11 Referenzen

Produktnummer ABIN2856661

Datenblatt (PDF)

SQSTM1 Antikörper

KO Validated SQSTM1 Reaktivität: Human WB, IF, IP, IHC (p), FACS, ICC, PLA Wirt: Kaninchen Polyclonal unconjugated

WB Image SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein by western blot analysis.

IHC-P Image SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein at cytoplasm on human ovarian carcinoma by immunohistochemical analysis.

ICC/IF Image SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein at autophagosome by immunofluorescent analysis.

WB Image SQSTM1 antibody detects SQSTM1 protein by western blot analysis.

WB Image Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE antibody diluted at 1:1000

WB Image SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein by Western blot analysis.

IP Image Immunoprecipitation of SQSTM1 protein from HeLa whole cell extracts using 5 μg of SQSTM1 antibody [N3C1], Internal, Western blot analysis was performed using SQSTM1 antibody [N3C1], Internal, EasyBlot anti-Rabbit IgG was used as a secondary reagent.

FACS Image SQSTM1 antibody [N3C1], Internal , detects SQSTM1 protein by flow cytometry analysis.

ICC/IF Image SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein at autophagosome by immunofluorescent analysis.

Analyses of GMI, TG and TM regulated ER stress, apoptosis and autophagy by Western blotting.

Analyses of GMI, TG and TM regulated ER stress, apoptosis and autophagy by Western blotting.

Analyses of GMI, TG and TM regulated ER stress, apoptosis and autophagy by Western blotting.

Analyses of GMI, TG and TM regulated ER stress, apoptosis and autophagy by Western blotting.

Analyses of GMI, TG and TM regulated ER stress, apoptosis and autophagy by Western blotting.

Analyses of GMI, TG and TM regulated ER stress, apoptosis and autophagy by Western blotting.

PB induces autophagy but blocks autophagosome trafficking and autophagosome-lysosome fusion in Hep3B cells.

HDAC2 positively regulates the expression of p-Akt, p-mTOR, and survivin in ER+ breast cancer cells.

Effect of autophagy on miR-21-induced drug resistance.

Effect of autophagy on miR-21-induced drug resistance.

Effect of autophagy on miR-21-induced drug resistance.

Effect of autophagy on miR-21-induced drug resistance.

Celastrol activates autophagy and inhibits MPP+-induced neurotoxicity in dopaminergic neurons.

Celastrol enhances the clearance of damaged mitochondria via activating mitophagy.

23 Abbildungen

13 Referenzen

Produktnummer ABIN2854836

Datenblatt (PDF)

CD25 Antikörper (AA 201-268)

IL2RA Reaktivität: Human, Maus FACS, WB, ELISA, IHC (p) Wirt: Kaninchen Polyclonal unconjugated

Immunohistochemistry validation image for anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 201-268) antibody (ABIN670371)

Immunohistochemistry validation image for anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 201-268) antibody (ABIN670371)

Lane 1: EC9706 lysates Lane 2: mouse lymph node lysates probed with IL2RA Polyclonal Antibody, Unconjugated at 1:500 dilution and 4˚C overnight incubation.

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-IL2RA Polyclonal Antibody, Unconjugated (ABIN670371) at 1:200 followed by conjugation to the secondary antibody and DAB staining

4 Abbildungen

Independent Validation

1 Validierung

Produktnummer ABIN670371

Datenblatt (PDF)

Independently Validated

PALB2 Antikörper (AA 1101-1186)

PALB2 Reaktivität: Human, Schwein WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

Western Blotting validation image for anti-Partner and Localizer of BRCA2 (PALB2) (AA 1101-1186) antibody (ABIN670536)

Images provided by the Independent Validation Program (badge number 29749).

Formalin-fixed and paraffin embedded human breast carcinoma labeled with Rabbit Anti PALB2 Polyclonal Antibody, Unconjugated (ABIN670536) at 1:200 followed by conjugation to the secondary antibody and DAB staining

HeLa cells probed with PALB2 Polyclonal Antibody, Unconjugated at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

4 Abbildungen

Independent Validation

1 Validierung

Produktnummer ABIN670536

Datenblatt (PDF)

Independently Validated

MEK2 Antikörper (AA 1-50)

MAP2K2 Reaktivität: Human, Maus WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

Western Blotting validation image for anti-Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (AA 1-50) antibody (ABIN726500)

Images provided by the Independent Validation Program, badge number 029748.

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti MEK2/MAPKK2 Polyclonal Antibody, Unconjugated (ABIN726500) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

HT-55 cells were stained with bs-0223R Rabbit Anti-MEK2 Polyclonal Antibody at 1:250.

4 Abbildungen

Independent Validation

1 Validierung

Produktnummer ABIN726500

Datenblatt (PDF)

Independently Validated

FOXO1 Antikörper (AA 165-270)

FOXO1 Reaktivität: Human, Maus, Ratte, Rind (Kuh), Schwein, Hund, Huhn WB, ELISA, IF (p), IHC (p), FACS Wirt: Kaninchen Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-Forkhead Box O1 (FOXO1) (AA 165-270) antibody (ABIN1387620)

Cleavage Under Targets and Release Using Nuclease validation image for anti-Forkhead Box O1 (FOXO1) (AA 165-270) antibody (ABIN1387620)

Formalin-fixed and paraffin embedded human bladder carcinoma labeled with Rabbit Anti FOXO1A Polyclonal Antibody, Unconjugated (ABIN1387620) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Antigen: 0.

4 Abbildungen

Independent Validation

1 Validierung

Produktnummer ABIN1387620

Datenblatt (PDF)

Independently Validated

BAD Antikörper (AA 101-204)

BAD Reaktivität: Human, Maus, Ratte WB, ELISA, IHC (p), IF (p), IF (cc), FACS, ICC, IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

Western Blotting validation image for anti-BCL2-Associated Agonist of Cell Death (BAD) (AA 101-204) antibody (ABIN674709)

Lane 1:Mouse kidney lysate Lane 2: Mouse brain lysate probed with Rabbit Anti-Bad Polyclonal Antibody, Unconjugated (ABIN674709) at 1:300 overnight at 4 °C.

Formalin-fixed and paraffin embedded mouse stomach labeled with Anti-Bad Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining

Image provided by the Independent Validation Program (badge number 29802).

4 Abbildungen

Independent Validation

1 Validierung

Produktnummer ABIN674709

Datenblatt (PDF)

Independently Validated

ALX4 Antikörper (AA 249-275)

ALX4 Reaktivität: Human WB, FACS, CUT&RUN Wirt: Kaninchen Polyclonal RB28072 unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-ALX Homeobox 4 (ALX4) (AA 249-275) antibody (ABIN2844113)

Cleavage Under Targets and Release Using Nuclease validation image for anti-ALX Homeobox 4 (ALX4) (AA 249-275) antibody (ABIN2844113)

ALX4 Antibody (Center) (ABIN654344 and ABIN2844113) western blot analysis in MDA-M cell line lysates (35 μg/lane).

ALX4 Antibody (Center) (ABIN654344 and ABIN2844113) flow cytometric analysis of MDA-M cells (right histogram) compared to a negative control cell (left histogram).

4 Abbildungen

Independent Validation

1 Validierung

Produktnummer ABIN654344

Datenblatt (PDF)

Independently Validated

BrdU Antikörper

BrdU FACS, IHC (p), ICC, IHC (fro) Wirt: Maus Monoclonal Bu20a unconjugated

Immunohistochemistry validation image for anti-Bromodeoxyuridine (BrdU) antibody (ABIN2477713)

Immunohistochemistry validation image for anti-Bromodeoxyuridine (BrdU) antibody (ABIN2477713)

Flow Cytometry (FACS) image for anti-Bromodeoxyuridine (BrdU) antibody (ABIN2477713)

3 Abbildungen

1 reference

Independent Validation

2 validations

Produktnummer ABIN2477713

Datenblatt (PDF)

Independently Validated

ICAM1 Antikörper

ICAM1 Reaktivität: Human, Ratte, Rind (Kuh) WB, FACS, ELISA, IHC (p), ICC Wirt: Maus Monoclonal MEM-111 unconjugated

Western blotting analysis of human CD54 using mouse monoclonal antibody MEM-111 on lysates of TF-1 and Raji cells, as well as of JURKAT cells (negative control) under reducing and non-reducing conditions.

Western blottin analysis of CD54 expression in TNF-alpha activated (A) and nonactivated (B) HUVEC cells by antibody MEM-111.

Flow cytometry analysis (surface staining) of CD54 expression in activated human peripheral blood with anti-CD54 (MEM-111) PE.

Flow cytometry multicolor surface staining pattern of human peripheral blood mononuclear cells using anti-human CD14 (MEM-15) PE antibody (20 μL reagent / 100 μL of peripheral whole blood) and anti-human CD54 (MEM-111) purified antibody (concentration in sample 0.

Separation of human monocytes (red-filled) from human lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral blood stained using anti-human CD54 (MEM-111) purified antibody (concentration in sample 0.

Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD54 (MEM-111) purified antibody (concentration in sample 0.

6 Abbildungen

7 Referenzen

Produktnummer ABIN94177

Datenblatt (PDF)

pan Keratin Antikörper

panKRT Reaktivität: Säugetier FACS, WB, IHC (p), ICC, IP Wirt: Maus Monoclonal C-11 unconjugated

Detection of cytokeratin on paraffin-embedded sections of guinea pig breast carcinoma using anti-cytokeratin antibody

Flow cytometry intracellular staining pattern of human peripheral whole blood spiked with MCF-7 cells stained using anti-Cytokeratins (C-11) purified antibody (concentration in sample 3 μg/mL, GAM APC).

Immunocytochemistry staining of cytokeratins in Hep-2 cells using pan-cytokeratin antibody C-11 ((ABIN94275), diluted 1:400), detected with GAM IgG-Alexa Fluor488 (diluted 1:200, green).

Immunohistochemistry staining of cytokeratin on paraffin-embedded sections of guinea pig breast carcinoma using anti-cytokeratin antibody (C-11).

Separation of MCF-7 cells (red-filled) from human leukocytes (black-dashed) in flow cytometry analysis (intracellular staining) of peripheral whole blood spiked with MCF-7 cells stained using anti-Cytokeratins (C-11) purified antibody (concentration in sample 3 μg/mL, GAM APC).

Western blotting analysis of human cytokeratins using mouse monoclonal antibody C-11 on lysates of HeLa cell line and MOLT-4 cell line (cytokeratin non-expressing cell line, negative control) under non-reducing and reducing conditions.

6 Abbildungen

6 Referenzen

Produktnummer ABIN94275

Datenblatt (PDF)

Integrin beta 2 Antikörper

ITGB2 Reaktivität: Human FACS, WB, IP, IHC (fro), IHC (p) Wirt: Maus Monoclonal MEM-48 unconjugated

Fig.

Immunohistochemistry staining of human spleen (paraffin sections) using anti-CD18 (MEM-48).

Immunohistochemistry staining of human spleen (paraffin sections) using anti-CD18 (Commercially tested by LifeSpan BioSciences.

Western Blotting analysis (non-reducing conditions) of whole cell lysate using anti-human CD18 ().

Separation of human leukocytes (red-filled) from CD18 negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD18 (MEM-48) purified antibody (concentration in sample 1 μg/mL, GAM APC).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD18 (MEM-48) purified antibody (concentration in sample 1 μg/mL, GAM APC).

6 Abbildungen

5 Referenzen

Produktnummer ABIN94005

Datenblatt (PDF)

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