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FACS Antikörper

Fluoreszenz-aktivierte Zellsortierung (FACS) ist eine spezielle Art der Durchflusszytometrie. Sie sortiert ein heterogenes Gemisch biologischer Zellen in zwei oder mehr Behälter, jeweils eine Zelle nach der anderen, auf der Grundlage der spezifischen Lichtstreuungs- und Fluoreszenzeigenschaften jeder Zelle und der an sie gebundenen Antikörper.

Wichtige Targets

Arginase 1 Antibodies

CASP8 Antibodies

CCR2 Antibodies

CD14 Antibodies

FOXP3 Antibodies

IL17 Antibodies

Lamin B1 Antibodies

Alle Flow Cytometry Antibodies

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100.640 Treffer

Beclin 1 Antikörper (AA 201-330)

BECN1 Reaktivität: Human, Maus, Ratte WB, ELISA, ICC, FACS, IHC (p), IF (p), IF (cc), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

293T lyates probed with Rabbit Anti-Beclin 1 Polyclonal Antibody, Unconjugated at 1:300 in 4˚C overnight.

Lane 1: mouse brain lysates Lane 2: mouse intestine lysates probed with Anti BECN1/Beclin 1/ATG6 Polyclonal Antibody, Unconjugated (ABIN675384) at 1:200 in 4C.

Formalin-fixed and paraffin embedded rat kidney labeled with Anti-Beclin 1/ATG6 Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin-embedded rat stomach labeled with Anti-Beclin 1/ATG6 Polyclonal Antibody, Unconjugatedused at 1:200 dilution for 40 minutes at 37°C.

Formalin-fixed and paraffin embedded rabbit myocardium labeled with Anti-BECN1/Beclin 1/ATG6 Polyclonal Antibody, Unconjugated (ABIN675384) followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded rat brain labeled with Anti-Beclin 1/ATG6 Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining

Image provided by One World Lab validation program.

Image provided by One World Lab validation program.

8 Abbildungen

11 Referenzen

Produktnummer ABIN675384

NRF2 Antikörper (pSer40)

NFE2L2 Reaktivität: Human, Maus, Ratte, Schwein WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

Formalin-fixed and paraffin embedded rat kidney tissue labeled with Anti-phospho-Nrf2 (Ser40) Polyclonal Antibody (ABIN676673), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded rat kidney tissue labeled with Anti-phospho-Nrf2 (Ser40) Polyclonal Antibody , Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded rat tongue tissue labeled with Anti-phospho-Nrf2 (Ser40) Polyclonal Antibody (ABIN676673), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded rat tongue tissue labeled with Anti-phospho-Nrf2 (Ser40) Polyclonal Antibody , Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded human colon carcinoma tissue labeled with Anti-phospho-Nrf2 (Ser40) Polyclonal Antibody (ABIN676673), Unconjugated 1:200 followed by conjugation to the secondary Cy5 antibody and DAPIstaining

L1 mouse heart, L2 mouse muscle lysates probed (ABIN676673) at 1:200 in 4 °C.

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-phospho-Nrf2 (Ser40) Polyclonal Antibody, Unconjugated (ABIN676673) at 1:200 followed by conjugation to the secondary antibody and DAB staining

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19 Referenzen

Produktnummer ABIN676673

Interferon gamma Antikörper (AA 75-155)

IFNG Reaktivität: Maus, Ratte WB, ELISA, FACS, IHC (p), IHC (fro), IF (p), IF (cc) Wirt: Kaninchen Polyclonal unconjugated

Diseased mouse lung and spleen lysates probed with Anti-IFN gamma Polyclonal Antibody at 1:5000 90min in 37˚C.

Antigen: 0.

Formalin-fixed and paraffin embedded rat lung tissue labeled with Anti-IFN-gamma Polyclonal Antibody, Unconjugated (ABIN669126) at 1:200, followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded rat lung tissue labeled with Anti-IFN-gamma Polyclonal Antibody, Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining

Mice were anesthetized and then infected intranasally by dropping 0.

Mice were anesthetized and then infected intranasally by dropping 0.

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21 Referenzen

Produktnummer ABIN669126

MMP2 Antikörper (AA 31-109)

MMP2 Reaktivität: Human, Ratte, Maus, Kaninchen WB, ELISA, FACS, IHC (p), IF (p), IF (cc), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

Paraformaldehyde-fixed, paraffin embedded rat brain, Antigen retrieval by boiling in sodium citrate buffer (pH6.

Formalin-fixed and paraffin embedded rat carotid artery Anti-MMP-2 Polyclonal Antibody , Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Mouse lung, Antigen retrieval by boiling in sodium citrate buffer (pH6.

HT1080 lysates probed with MMP-2 Polyclonal Antibody, Unconjugated at 1:300 dilution and 4˚C overnight incubation.

HeLa cells probed with MMP2 Polyclonal Antibody, unconjugated at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange)

Paraformaldehyde-fixed, paraffin embedded Rat Brain Antigen retrieval by boiling in sodium citrate buffer (pH6.

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17 Referenzen

Produktnummer ABIN668286

PTGS2 Antikörper (AA 501-604)

PTGS2 Reaktivität: Human, Maus, Ratte WB, ELISA, IHC (p), IF (cc), IF (p), FACS, IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

Mouse brain lysates probed with Rabbit Anti-Cyclooxygenase 2 Polyclonal Antibody, Unconjugated at 1:5000 for 90 min at 37˚C.

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti Cyclooxygenase 2/COX2 Polyclonal Antibody, Unconjugated (ABIN672471) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Antigen: 0.

Human A549 lysates probed with Rabbit Anti-Cyclooxygenase 2 Polyclonal Antibody, Unconjugated at 1:5000 for 90 min at 37˚C.

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti Cyclooxygenase 2/COX2 Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining

Antigen: 0.

Effects of Celecoxib on COX-2 and cyclin D1 activation in SKOV-3 xenograft tumors: (A) Western blot analysis of SKOV-3 tumors treated with a high-dose of Celecoxib (100 mg/kg).

COX protein levels in xenograft tumors of nude mice treated or not treated with combined treatment of SC-560 and celecoxib.

COX-2 over expressed in MDR-Caco-2 cells, which could be reversed by sinomenine.

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14 Referenzen

Produktnummer ABIN672471

HIF1A Antikörper (AA 341-450)

HIF1A Reaktivität: Human, Maus, Ratte, Kaninchen WB, ELISA, IP, FACS, IHC (p), IF (p), IF (cc), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

HL-60 cell lysates probed with Anti-HIF-1 Alpha Polyclonal Antibody, Unconjugated at 1:5000 for 90 min at 37˚C.

Formalin-fixed and paraffin embedded human cervical carcinoma labeled Anti-HIF-1-Alpha Polyclonal Antibody (ABIN672546), Unconjugated at 1:300, followed by conjugation to the secondary antibody and DAB staining

Image kindly submitted by Edita Aksamitiene as part of the Free Sample Program.

HeLa cells probed with HIF-1 Alpha Polyclonal Antibody, unconjugated at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange)

L1 mouse heart lysates, L2 mouse large intestine lysates probed with Anti HIF-1 Alpha Polyclonal Antibody, Unconjugated (ABIN672546) at 1:200 in 4 °C.

KLN 205 cells labeled with Rabbit HIF-1 Alpha Polyclonal Antibody Polyclonal Antibody, Unconjugated followed by a FITC Conjugated Secondary Antibody.

Formalin-fixed and paraffin embedded human cervical carcinoma labeled Anti-HIF-1-Alpha Polyclonal Antibody , Unconjugated at 1:300, followed by conjugation to the secondary antibody and DAB staining

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10 Referenzen

Produktnummer ABIN672546

Cyclin D1 Antikörper (AA 61-110)

CCND1 Reaktivität: Human, Maus, Ratte, Rind (Kuh), Kaninchen, Schaf WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

A549 cells probed with Anti-Cyclin D1 Polyclonal Antibody at 1:5000 90min in 37˚C.

Formalin-fixed and paraffin embedded human placenta tissue labeled with Anti-Cyclin D1 Polyclonal Antibody, Unconjugated (ABIN671016) at 1:200, followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded human placenta tissue labeled with Anti-Cyclin D1 Polyclonal Antibody, unconjugated (ABIN671016) at 1: 200, followed by incubation with conjugated secondary antibody and DAB staining

RSC96 cells probed with Cyclin D1 Polyclonal Antibody, Unconjugated at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

Image provided by One World Lab validation program.

Image provided by One World Lab validation program.

6 Abbildungen

15 Referenzen

Produktnummer ABIN671016

Collagen Type I Antikörper (AA 1321-1400)

COL1 Reaktivität: Human, Maus, Ratte, Kaninchen, Fisch, Hund WB, ELISA, IHC (fro), IHC (p), IF (cc), IF (p), FACS Wirt: Kaninchen Polyclonal unconjugated

SW480 lysates probed with Rabbit Anti-Pro-Collagen I Polyclonal Antibody, Unconjugated at 1:5000 for 90 min at 37˚C.

Formalin-fixed and paraffin-embedded rabbit meniscus tissue labeled with Anti-Collagen I Polyclonal Antibody, Unconjugated (ABIN670386) 1:300 followed by conjugation to the secondary PE antibody and DAB staining

Formalin-fixed and paraffin-embedded dog bladder tissue labeled with Anti-Collagen I Polyclonal Antibody, Unconjugated (ABIN670386) 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin-embedded rat colon labeled with Anti-Collagen I Polyclonal Antibody, Unconjugated (ABIN670386) 1:200 followed by conjugation to the secondary antibody and DAB staining

Mouse muscle lysates probed with Rabbit Anti-Pro-Collegen I Polyclonal Antibody, Unconjugated at 1:5000 for 90 min at 37˚C.

Formalin-fixed and paraffin-embedded rat colon labeled with Anti-Collagen I Polyclonal Antibody, Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin-embedded dog bladder tissue labeled with Anti-Collagen I Polyclonal Antibody, Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining

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16 Referenzen

Produktnummer ABIN670386

CD31 Antikörper (AA 601-680)

PECAM1 Reaktivität: Human, Maus, Hund FACS, WB, ELISA, IHC (p), IHC (fro), IF (p), IF (cc) Wirt: Kaninchen Polyclonal unconjugated

Recombinant human CD31 protein probed with CD31 Polyclonal Antibody, unconjugated at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.

Formalin-fixed and paraffin embedded rat skin tissue labeled with Anti-CD31/PECAM-1 Polyclonal Antibody, Unconjugated at 1:300, followed by conjugation to the secondary antibody and DAB staining

Human Jurkat cells probed with CD31 Polyclonal Antibody, Unconjugated (green) at 1:100 for 30 minutes followed by a FITC conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).

Formalin-fixed and paraffin embedded rat skin tissue labeled with Anti-CD31/PECAM-1 Polyclonal Antibody, Unconjugated (ABIN669006) at 1:300, followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed: Endothelial cells labeled with Anti-CD31/PECAM-1 Polyclonal Antibody, Unconjugated (ABIN669006) at 1:200, followed by conjugation to the secondary antibody was Goat Anti-Rabbit IgG, FITC conjugated at 1:200 for 40 minutes at 37°

Formalin-fixed and paraffin embedded rat brain labeled with Anti-CD31 Polyclonal Antibody, Unconjugated at 1:300 followed by conjugation to the secondary antibody and DAB staining.

Rat spleen cells probed with Rabbit Anti-CD31 Polyclonal Antibody Alexa Fluor 488 Conjugated Secondary Antibody.

Formalin-fixed and paraffin embedded rat brain labeled with Anti-CD31 Polyclonal Antibody, Unconjugated at 1:300 followed by conjugation to the secondary antibody and DAB staining.

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16 Referenzen

Produktnummer ABIN669006

alpha Tubulin Antikörper (FITC)

TUBA1 Reaktivität: Human, Maus, Schwein, Saccharomyces cerevisiae, Arabidopsis, Nicotiana tabacum, Eisenia fetida, Paramecium, Pute ICC, FACS Wirt: Maus Monoclonal TU-01 FITC

Use of anti-alpha-tubulin antibody as a loading control (A) in an Western blotting experiment revealing the staining pattern ofvarious cell lysates by a newly developed monoclonal antibody (B).

Separation of HeLa cells stained using anti-human alpha-tubulin (TU-01) FITC antibody (concentration in sample 5 μg/mL, red-filled) from HeLa cells stained using mouse IgG1 isotype control (MOPC-21) FITC antibody (concentration in sample 5 μg/mL, same as alpha-tubulin FITC concentration, black-dashed) in flow cytometry analysis (intracellular staining) of HeLa cell suspension.

Use of anti-alpha-tubulin antibody as a loading control (A) in an Western blotting experiment revealing the staining pattern ofvarious cell lysates by a newly developed monoclonal antibody (B).

Nucleus is stained with DAPI (blue).

Immunofluorescence staining of HeLa human cervix carcinoma cell line using anti-alpha-tubulin (; red).

Immunofluorescence staining of 3T3 mouse embryonal fibroblast cell line using anti-alpha-tubulin (TU-01 ; green) and anti-Vimentin (VI-01; red).

Immunocytochemistry staining of alpha tubulin in HeLa cells using mouse monoclonal antibody TU-01 FITC.

Immunocytochemistry staining of alpha tubulin in HeLa cells using mouse monoclonal antibody TU-01 FITC.

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11 Referenzen

Produktnummer ABIN93892

TUBB3 Antikörper (N-Term) (FITC)

TUBB3 Reaktivität: Human, Maus, Ratte, Hund, Schwein FACS, ICC Wirt: Maus Monoclonal TU-20 FITC

Immunofluorescence staining (mouse neuroblastoma cells) Immunofluorescence staining of Neuro2a mouse neuroblastoma cell line using anti-betaIII-tubulin (TU-20 ; green; 3 μg/ml).

Separation of MCF-7 cells stained using anti-human betaIII-Tubulin (TU-20) FITC antibody (10 μL reagent per million cells in 100 μL of cell suspension, red-filled) from MCF-7 cells stained using mouse IgG1 isotype control (MOPC-21) FITC antibody (concentration in sample 15 μg/mL, same as betaIII-Tubulin FITC concentration, black-dashed) in flow cytometry analysis (intracellular staining) of MCF-7 cell suspension.

Immunofluorescence staining of P-19 mouse embryonal carcinoma cell line stimulated to neuronal differentiation by retinoic acid.

Immunofluorescence staining (mouse embryonal carcinoma cells) Immunofluorescence staining of P-19 mouse embryonal carcinoma cell line stimulated to neuronal differentiation by retinoic acid.

Immunofluorescence staining of Neuro2a mouse neuroblastoma cell line using anti-betaIII-tubulin (TU-20 FITC, green, 1 μg/ml).

Immunofluorescence staining of Neuro2a mouse neuroblastoma cell line using anti-betaIII-tubulin (TU-20 FITC, green, 1 μg/mL).

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10 Referenzen

Produktnummer ABIN93912

beta Actin Antikörper

ACTB Reaktivität: Human, Maus, Ratte WB, IF, IHC (p), FACS, DNA Mic Wirt: Maus Monoclonal 137CT26-1-1 unconjugated

All lanes : Anti-ACTB Antibody at 1:2000 dilution + C6 cell lysate Lysates/proteins at 20 μg per lane.

Immunohistochemical analysis of paraffin-embedded H.

Confocal immunofluorescent analysis of ACTB Antibody (ABIN658990 and ABIN2838035) with Hela cell followed by Alexa Fluor® 488-conjugated goat anti-mouse lgG (green).

All lanes : Anti-ACTB Antibody at 1:1000 dilution Lane 1: A431 whole cell lysate Lane 2: C2C12 whole cell lysate Lane 3: C6 whole cell lysate Lane 4: Hela whole cell lysate Lane 5: MCF-7 whole cell lysate Lysates/proteins at 20 μg per lane.

Western blot analysis of anti-ACTB Antibody (ABIN658990 and ABIN2838035) in K562, HL-60,Hela cell line, mouse spleen, mouse liver tissue lysates, mouse NIH-3T3 cell line lysate and mouse cerebellum, mouse brain tissue lysates (35 μg/lane).

All lanes : Anti-ACTB Antibody at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: NIH-3T3 whole cell lysate Lysates/proteins at 20 μg per lane.

(ABIN658990 and ABIN2838035) staining ACTB in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

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12 Referenzen

Produktnummer ABIN658990

FcRn Antikörper

FcRn Reaktivität: Human ELISA, IF, FACS, cELISA, Func Wirt: Maus Monoclonal ADM31 unconjugated

Immunofluorescence: huSSECTM cell line overexpressing hFcRn-EGFP using clone ADM31 at 15µg/ml and Texas Red™ conjugated anti-mouse secondary.

Antibody- or peptide-mediated disruption of the human FcRn–albumin interactions decreases chemical hepatotoxicity.

293hFcRn-GFP cells were incubated with a range of concentrations of DVN1, ADM11, DVN21, DVN22, DVN23, DVN24, ADM31 or ADM32 at pH 6, then stained for functional binding with (A) 20 μg/mL hIgGAF647, or (B) 200 μg/mL HSAbiotin followed by streptavidin-PE.

Tracer plasma concentrations were determined by ELISA and plotted either as percent remaining as compared with the first time point plasma concentrations or as plasma concentrations ±SD.

ELISA binding of human, macaque, pig, dog, mouse, and rat FcRn toward ADM31 at pH 7.

Identification of monoclonal Abs that block albumin binding to hFcRn.

Immunofluorescence staining of huSSECTM cell line overexpressing hFcRn-EGFP using clone ADM31 at 15µg/ml and Texas Red™ conjugated anti-mouse secondary.

Flowcytometry of huSSECTM cell line overexpressing hFcRn-EGFP labeled with clone ADM31 primary Ab and Texas Red™ conjugated anti-mouse secondary.

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16 Referenzen

Produktnummer ABIN1774762

SQSTM1 Antikörper

KO Validated SQSTM1 Reaktivität: Human WB, IF, ICC, IP, FACS, IHC (p), PLA Wirt: Kaninchen Polyclonal unconjugated

WB Image SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein by western blot analysis.

IHC-P Image SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein at cytoplasm on human ovarian carcinoma by immunohistochemical analysis.

ICC/IF Image SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein at autophagosome by immunofluorescent analysis.

WB Image SQSTM1 antibody detects SQSTM1 protein by western blot analysis.

WB Image Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE antibody diluted at 1:1000

WB Image SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein by Western blot analysis.

IP Image Immunoprecipitation of SQSTM1 protein from HeLa whole cell extracts using 5 μg of SQSTM1 antibody [N3C1], Internal, Western blot analysis was performed using SQSTM1 antibody [N3C1], Internal, EasyBlot anti-Rabbit IgG was used as a secondary reagent.

FACS Image SQSTM1 antibody [N3C1], Internal , detects SQSTM1 protein by flow cytometry analysis.

ICC/IF Image SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein at autophagosome by immunofluorescent analysis.

Analyses of GMI, TG and TM regulated ER stress, apoptosis and autophagy by Western blotting.

Analyses of GMI, TG and TM regulated ER stress, apoptosis and autophagy by Western blotting.

Analyses of GMI, TG and TM regulated ER stress, apoptosis and autophagy by Western blotting.

Analyses of GMI, TG and TM regulated ER stress, apoptosis and autophagy by Western blotting.

Analyses of GMI, TG and TM regulated ER stress, apoptosis and autophagy by Western blotting.

Analyses of GMI, TG and TM regulated ER stress, apoptosis and autophagy by Western blotting.

PB induces autophagy but blocks autophagosome trafficking and autophagosome-lysosome fusion in Hep3B cells.

HDAC2 positively regulates the expression of p-Akt, p-mTOR, and survivin in ER+ breast cancer cells.

Effect of autophagy on miR-21-induced drug resistance.

Effect of autophagy on miR-21-induced drug resistance.

Effect of autophagy on miR-21-induced drug resistance.

Effect of autophagy on miR-21-induced drug resistance.

Celastrol activates autophagy and inhibits MPP+-induced neurotoxicity in dopaminergic neurons.

Celastrol enhances the clearance of damaged mitochondria via activating mitophagy.

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13 Referenzen

Produktnummer ABIN2854836

Liver Arginase Antikörper

KO Validated ARG1 Reaktivität: Human WB, ELISA, IF, IP, FACS, IHC (p), ICC, IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

WB Image Sample (20 ug of whole cell lysate) A: mouse liver 10% SDS PAGE antibody diluted at 1:50000

ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using arginase I , antibody at 1:200 dilution.

IHC-P Image Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using arginase I , antibody at 1:500 dilution.

WB Image Arginase 1 antibody detects Arginase 1 protein by western blot analysis.

Elevated right ventricular pressure, increased right ventricular weight and altered expression of Ddah1 and Arg1 in cavin-/- mice.

Elevated arginase1 expression and increased arginase inhibitor-induced relaxation of Cavin-1-/- arteries.

Elevated arginase1 expression and increased arginase inhibitor-induced relaxation of Cavin-1-/- arteries.

Arg-1 expression in neutrophils and lymph-node microenvironmentNeutrophils isolated from control and HL patients were analysed for Arg-1 expression (A), activity by measuring urea (B) and protein expression by Western Blot (C).

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11 Referenzen

Produktnummer ABIN2856661

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