GST Antikörper (Alkaline Phosphatase (AP))

Produktnummer ABIN99863

Hersteller Produkt- Nr.: 600-105-200

Produktdetails anti-GST Antikörper (Alkaline Phosphatase (AP))

Target: GST (Glutathione S Transferase (GST))

Reaktivität: Schistosoma japonicum

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser GST Antikörper ist konjugiert mit Alkaline Phosphatase (AP)

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Hersteller Produkt- Nr.: 600-105-200

Hersteller: Rockland

Verwendungszweck: GST Antibody Alkaline Phosphatase Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Alkaline Phosphatase, as well as purified and partially purified Glutathione-S-Transferase [Schistosoma japonicum].

Produktmerkmale: Synonyms: goat anti-GST antibody alkaline phosphatase conjugation, alk phos conjugated goat anti-GST antibody, Glutathione-S-Transferase, GST antibody, anti-GST antibody, anti-Glutathione-S-Transferase antibody, ALP, Alk Phos

Aufreinigung: Anti-GST Alkaline Phosphatase Conjugated antibody as prepared from monospecific antiserum by immunoaffinity chromatography using GST coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Glutathione-S-Transferase [Schistosoma japonicum]

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:200 - 1:1,000

Application Note: Anti-GST Antibody Alkaline Phosphatase conjugated has been tested by ELISA and western blot and is suitable for immunoblotting, ELISA, immunohistochemistry as well as other antibody based assays using alkaline phosphatase conjugates requiring lot-to-lot consistency. Optimal concentrations in these or other immunoassays should be determined by the researcher.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:1,000 - 1:5,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.1 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Haltbarkeit: 12 months

Details zu GST

Target: GST (Glutathione S Transferase (GST))

Andere Bezeichnung: GST (GST Produkte)

Hintergrund: Background: GST Antibody is specific for the GST affinity tag. Affinity tags re appended to proteins thereby allowing them to be purified from their crude biological source using an affinity technique. Common affinity tags include glutathione-S-transferase (GST), chitin binding protein (CBP), maltose binding protein (MBP), and the poly-Histidine or HIS-tag.