GLB1 Antikörper (HRP)

Produktnummer ABIN99529

Produktdetails anti-GLB1 Antikörper (HRP)

Target: GLB1 (Galactosidase, beta 1 (GLB1))

Reaktivität: E. coli

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GLB1 Antikörper ist konjugiert mit HRP

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Dot Blot (DB), Labeling (Lbl), Fluorescence Microscopy (FM), Conjugation (Con)

Produktmerkmale: Concentration Definition: by UV absorbance at 280 nm

Immunogen: Beta Galactosidase (E.coli)
Immunogentype:Native

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Suitable for immunoblotting (western or dot blot), ELISA, immunofluorescence microscopy, immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity. The antibody recognizes both frozen tissue sections, paraffin embedded tissue and 4% paraformaldehyde fixed tissue for most immunohistochemical analysis. A 1:5,000 dilution has been reported to be successful for staining by immunoblot of beta-galactosidase fusion proteins after transfer using a semi-dry transfer apparatus.  A 1:1,500 dilution has been reported to detect beta-galactosidase in adult rat spinal cord tissue after infection with helper-dependent adenovirus expressing lacZ.  In this particular experiment, tissue was perfused with 4% paraformaldehyde and cryostat-cut (35 µm) to produce free-floating sections.  A 1:5,000 dilution has been reported to be successful for staining of brain sections from transgenic mice expressing nuclear beta-galactosidase when assayed by immunofluorescence microscopy.  A 1:5,000 dilution has been reported for immunofluorescent staining of methanol fixed, devitellinized Drosophila embryos.  Although a wide range of conditions was reported to be effective, a 1:10,000 dilution was noted to show no background and to be suitable for double labeling experiments.  Optimal titers for other applications should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Restore with deionized water (or equivalent)

Konzentration: 10.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Konservierungsmittel: Gentamicin sulfate

Handhabung: Do NOT add Sodium Azide!

Lagerung: 4 °C

Details zu GLB1

Target: GLB1 (Galactosidase, beta 1 (GLB1))

Andere Bezeichnung: Beta Galactosidase (GLB1 Produkte)

Synonyme: EBP antikoerper, ELNR1 antikoerper, MPS4B antikoerper, DER antikoerper, XR antikoerper, 0610038K04Rik antikoerper, 1810027P18Rik antikoerper, glb antikoerper, CG9092 antikoerper, Dmel\\CG9092 antikoerper, beta-GAL antikoerper, beta-Gal-1 antikoerper, beta-gal antikoerper, gal antikoerper, lacZ-1 antikoerper, AW125515 antikoerper, Bge antikoerper, Bgl antikoerper, Bgl-e antikoerper, Bgl-s antikoerper, Bgl-t antikoerper, Bgs antikoerper, Bgt antikoerper, C130097A14Rik antikoerper, BGAL antikoerper, zgc:110823 antikoerper, ECK0341 antikoerper, JW0335 antikoerper, GLB antikoerper, galactosidase beta 1 antikoerper, dicarbonyl and L-xylulose reductase antikoerper, dicarbonyl L-xylulose reductase antikoerper, beta-D-galactosidase antikoerper, beta-galactosidase antikoerper, beta galactosidase antikoerper, galactosidase, beta 1 antikoerper, beta-galactosidase-like antikoerper, GLB1 antikoerper, DCXR antikoerper, Dcxr antikoerper, lacZ antikoerper, SSP_RS00525 antikoerper, lacH antikoerper, ECL_03691 antikoerper, Gal antikoerper, Glb1 antikoerper, glb1 antikoerper, VV2_1327 antikoerper, LOC100056371 antikoerper

Hintergrund: Synonyms: HRP

Pathways: Glycosaminoglycan Metabolic Process