Fluorescein Antikörper (Rhodamine)

Produktnummer ABIN99348

Hersteller Produkt- Nr.: 600-100-096

Dieses Anti--Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von in WB, FLISA und FM. Geeignet für .

Kurzübersicht für Fluorescein Antikörper (Rhodamine) (ABIN99348)

Target: Fluorescein

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Rhodamine

Applikation: Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)

Produktdetails

Hersteller Produkt- Nr.: 600-100-096

Hersteller: Rockland

Verwendungszweck: Fluorescein Antibody Rhodamine Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and Fluorescein Conjugated IgG.

Produktmerkmale: Synonyms: goat Anti-FITC antibody Rhodamine conjugation, TRITC conjugated goat anti-fluorescein antibody, TRITC conjugated goat Anti-FITC antibody, goat anti-fluorescein antibody Rhodamine conjugation

Aufreinigung: Fluorescein Antibody Conjugated to Rhodamine was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogen:

Immunogen: Fluorescein conjugated to Goat IgG

Immunogen Type: Other

Isotyp: IgG

Labeling Ratio: 2.0

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti-Fluorescein Rhodamine Conjugated Antibody is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.

Western Blot Dilution: >1:10,000

FLISA Dilution: >1:20,000

IF Microscopy Dilution: 1:500 - 1:2,500

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Antigendetails

Target: Fluorescein

Substanzklasse: Chemical

Hintergrund: Background: Anti-Fluorescein Rhodamine Conjugated Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.