Fluorescein Antikörper

Produktnummer ABIN5706816

Produktdetails

Target: Fluorescein

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC)

Produktmerkmale:

Synonyms: mouse Anti-FITC antibody, mouse anti-fluorescein antibody

Background: Anti-Fluorescein is designed for immunofluorescence microscopy, in situ hybridization, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Aufreinigung: Fluorescein Antibody was prepared from mouse ascites by Protein A chromatography. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-mouse serum, Fluorescein conjugated IgG and Fluorescein conjugated Bovine Serum Albumin.

Immunogen:

Immunogen: Fluorescein conjugated to Mouse IgG

Immunogen Type: Other

Klon: 8B9-C6-D3

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise:

Flow Cytometry Dilution: 1-2 μg/mL

Immunohistochemistry Dilution: 1:250 - 1:1,500

Application Note: Anti-Fluorescein Antibody is suitable for immunomicroscopy,in situ hybridization and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.

Western Blot Dilution: 1:500 - 1:3,000

ELISA Dilution: 1:5,000 - 1:30,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride,  pH 7.2

Stabilizer: None

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Haltbarkeit: 12 months

Referenzen

Kotani, Yamaguchi, Ohnishi, Kuwahara, Nakano, Nakano, Ida, Murakoshi, Honke: "Proximity proteomics identifies cancer cell membrane cis-molecular complex as a potential cancer target." in: Cancer science, Vol. 110, Issue 8, pp. 2607-2619, (2019) (PubMed).

Miyagawa-Yamaguchi, Kotani, Honke: "Expressed glycosylphosphatidylinositol-anchored horseradish peroxidase identifies co-clustering molecules in individual lipid raft domains." in: PLoS ONE, Vol. 9, Issue 3, pp. e93054, (2015) (PubMed).

Antigendetails

Target: Fluorescein

Substanzklasse: Chemical