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Fluorescein Antikörper (HRP)

WB, ELISA, IHC, DB Wirt: Ziege Polyclonal HRP
Rockland
Produktnummer ABIN99343
Hersteller Produkt- Nr.: 600-103-096
  • Target
    Fluorescein
    Wirt
    • 28
    • 18
    • 10
    • 8
    • 4
    • 2
    Ziege
    Klonalität
    • 29
    • 27
    • 14
    Polyklonal
    Konjugat
    • 45
    • 5
    • 4
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    HRP
    Applikation
    • 32
    • 22
    • 18
    • 18
    • 17
    • 13
    • 11
    • 10
    • 8
    • 6
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Dot Blot (DB)
    Hersteller Produkt- Nr.
    600-103-096
    Hersteller
    Rockland
    Verwendungszweck
    Fluorescein Antibody Peroxidase Conjugated
    Kreuzreaktivität (Details)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum and Fluorescein conjugated IgG.
    Produktmerkmale
    Synonyms: goat Anti-FITC antibody HRP conjugation, peroxidase conjugated goat anti-fluorescein antibody, peroxidase conjugated goat Anti-FITC antibody, goat anti-fluorescein antibody HRP conjugation
    Aufreinigung
    Fluorescein Antibody Peroxidase Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein coupled to sepharose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
    Immunogen

    Immunogen: Fluorescein conjugated to Goat IgG

    Immunogen Type: Other

    Isotyp
    IgG
  • Applikationshinweise

    Immunohistochemistry Dilution: 1:500 - 1:2,500

    Application Note: Anti-Fluorescein Antibody Peroxidase Conjugated has been tested by ELISA, dot blot, and western blot. This product is suitable for immunoblotting (western or dot blot), ELISA, and in situ hybridization, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.

    Western Blot Dilution: 1:5,000 - 1:10,000

    ELISA Dilution: 1:200,000

    Other: User Optimized

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution

    Reconstitution Volume: 1.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Konzentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
    Konservierungsmittel
    Gentamicin sulfate
    Vorsichtsmaßnahmen
    This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
    Haltbarkeit
    12 months
  • Target
    Fluorescein
    Substanzklasse
    Chemical
    Hintergrund
    Background: Anti-Fluorescein Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. Anti-FITC is also suitable as a visualization reagent for fluorescein-labelled compounds (such as antibodies, lectins and peptide nucleic acid (PNA) probes).
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