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Fetuin A Antikörper

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch Fetuin A in WB und ELISA. Er zeigt eine Reaktivität gegenüber Human.
Rockland
Produktnummer ABIN99178
Hersteller Produkt- Nr.: 200-101-456

Kurzübersicht für Fetuin A Antikörper (ABIN99178)

Target

Alle Fetuin A (AHSG) Antikörper anzeigen
Fetuin A (AHSG) (alpha-2-HS-Glycoprotein (AHSG))

Reaktivität

  • 99
  • 31
  • 26
  • 10
  • 5
  • 4
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
Human

Wirt

  • 100
  • 28
  • 8
  • 4
  • 1
  • 1
Ziege

Klonalität

  • 113
  • 27
Polyklonal

Konjugat

  • 89
  • 11
  • 7
  • 4
  • 4
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser Fetuin A Antikörper ist unkonjugiert

Applikation

  • 99
  • 63
  • 41
  • 39
  • 37
  • 23
  • 18
  • 11
  • 10
  • 7
  • 6
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), ELISA
  • Hersteller Produkt- Nr.

    200-101-456

    Hersteller

    Rockland

    Verwendungszweck

    Fetuin Antibody

    Kreuzreaktivität (Details)

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum.

    Produktmerkmale

    Synonyms: goat anti-Fetuin antibody, 59 kDa bone sialic acid-containing protein antibody, A2HS antibody, Aa2-066 antibody, AHS antibody, Ahsg alpha-2-HS-glycoprotein antibody, AHSG antibody, Alpha 2 Z globulin antibody, Alpha-2-HS-glycoprotein antibody, Ba-alpha-2-glycoprotein, Fetuin-A, PRO2743

    Aufreinigung

    This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

    Immunogen

    Immunogen: This purified antibody was prepared from rabbit serum after repeated immunizations with a recombinant human fetuin (a2-HS glycoprotein) processed to remove a 40 amino acid residue bridging peptide resulting in the mature form of the protein.

    Immunogen Type: Conjugated Peptide

    Isotyp

    IgG
  • Applikationshinweise

    Application Note: This purified polyclonal antibody has been tested by western blot. Although not tested, this antibody is likely functional in ELISA, immunohistochemistry, and immunoprecipitation.  A doublet band or slightly lower molecular weight band (see image) may be visible by western blot due to proteolytic processing, variable glycosylation and/or phosphorylation.  Proteolytic processing may include the removal of a 40 amino acid residue bridging peptide from the A and B chains of fetuin in vivo.

    Western Blot Dilution: 1:10,000 - 1:20,000

    ELISA Dilution: 1:40.000 - 1:200,000

    Other: User Optimized

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Reconstitution Volume: 500 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Konzentration

    1.0 mg/mL

    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

    Haltbarkeit

    12 months
  • Target

    Fetuin A (AHSG) (alpha-2-HS-Glycoprotein (AHSG))

    Andere Bezeichnung

    AHSG

    Hintergrund

    Background: Human fetuin (2-Heremans-Schmid-glycoprotein or α2-HS-glycoprotein) is a major plasma glycoprotein predominantly synthesized in the liver.  Human fetuin is named after its bovine homolog.  Fetuins are found in most mammals. Human fetuin is a negative acute-phase protein, normal circulating levels in adults (300-600 μg/mL) fall significantly (30-50 % ) during injury and infection. The biological role of fetuin is unknown, although it has been implicated as an immunomodulator that can participate in stimulation of bacterial phagocytosis by neutrophils and promotion of endocytosis by mouse macrophages. Hepatocytes are the principal cell source of circulating fetuin, but it also is expressed by monocyte/macrophages. Fetuins occur in large amounts in blood and cerebrospinal fluid and accumulate to high concentrations in calcified bone.  The fetuin promoter region has several potential interleukin 6-responsive elements, and its synthesis is down-regulated during injury and inflammation. Fetuin is an acidic glycoprotein with three N-linked and three O-linked oligosaccharide chains, whose terminal sugar residues are rich in sialic acid (N-acetylneuraminic acid), contributing to its net negative charge. A role for fetuin as a carrier of bioactive molecules has been proposed based on observations that it binds and carries Ca2+ ion.  Fetuin is implicated in bone remodeling, immune function and may play a role in tumor progression of certain cell types.

    Gen-ID

    197

    NCBI Accession

    NP_001613

    UniProt

    P02765
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