INCENP Antikörper (AA 369-583)

Produktnummer ABIN969529

Dieses Maus Monoklonal-Antikörper erkennt spezifisch INCENP in FACS, ELISA, ICC und IHC. Er zeigt eine Reaktivität gegenüber Human und wurde in 2+ Publikationen erwähnt.

Kurzübersicht für INCENP Antikörper (AA 369-583) (ABIN969529)

Target: INCENP (Inner Centromere Protein Antigens 135/155kDa (INCENP))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser INCENP Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (IHC)

Klon: 3D2

Produktdetails anti-INCENP Antikörper

Bindungsspezifität: AA 369-583

Verwendungszweck: INCENP Antibody

Aufreinigung: Purified antibody

Immunogen: Purified recombinant fragment of human INCENP (AA: 369-583) expressed in E. Coli.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

ELISA: 1/10000

FCM: 1/200 - 1/400

ICC: 1/200 - 1/1000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified antibody in PBS with 0.05 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Referenzen für anti-INCENP Antikörper (ABIN969529)

Ban, Nishida, Urano: "Mitotic kinase Aurora-B is regulated by SUMO-2/3 conjugation/deconjugation during mitosis." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 16, Issue 6, pp. 652-69, (2011) (PubMed).

Xu, Ogawa, Vagnarelli, Bergmann, Hudson, Ruchaud, Fukagawa, Earnshaw, Samejima: "INCENP-aurora B interactions modulate kinase activity and chromosome passenger complex localization." in: The Journal of cell biology, Vol. 187, Issue 5, pp. 637-53, (2009) (PubMed).

Details zu INCENP

Target: INCENP (Inner Centromere Protein Antigens 135/155kDa (INCENP))

Andere Bezeichnung: INCENP

Hintergrund: In mammalian cells, 2 broad groups of centromere-interacting proteins have been described: constitutively binding centromere proteins and 'passenger,' or transiently interacting, proteins (reviewed by Choo, 1997). The constitutive proteins include CENPA (centromere protein A, MIM 117139), CENPB (MIM 117140), CENPC1 (MIM 117141), and CENPD (MIM 117142). The term 'passenger proteins' encompasses a broad collection of proteins that localize to the centromere during specific stages of the cell cycle (Earnshaw and Mackay, 1994 [PubMed 8088460]). These include CENPE (MIM 117143), MCAK (MIM 604538), KID (MIM 603213), cytoplasmic dynein (e.g., MIM 600112), CliPs (e.g., MIM 179838), and CENPF/mitosin (MIM 600236). The inner centromere proteins (INCENPs) (Earnshaw and Cooke, 1991 [PubMed 1860899]), the initial members of the passenger protein group, display a broad localization along chromosomes in the early stages of mitosis but gradually become concentrated at centromeres as the cell cycle progresses into mid-metaphase. During telophase, the proteins are located within the midbody in the intercellular bridge, where they are discarded after cytokinesis

Molekulargewicht: 105.4 kDa

Gen-ID: 3619

UniProt: Q9NQS7

Pathways: Zellzyklus