Nanog Antikörper (AA 20-166)

Produktnummer ABIN969308

Der Maus Monoklonal Anti-Nanog-Antikörper wurde für WB, ELISA und ICC validiert. Er ist geeignet, Nanog in Proben von Human zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für Nanog Antikörper (AA 20-166) (ABIN969308)

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Nanog Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Klon: 1E6C4

Produktdetails anti-Nanog Antikörper

Bindungsspezifität: AA 20-166

Verwendungszweck: NANOG Antibody

Aufreinigung: Ascitic fluid

Immunogen: Purified recombinant fragment of NANOG (aa20-166) expressed in E. Coli.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

ELISA: 1/10000

ICC: 1/200 - 1/1000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Referenzen für anti-Nanog Antikörper (ABIN969308)

Freberg, Dahl, Timoskainen, Collas: "Epigenetic reprogramming of OCT4 and NANOG regulatory regions by embryonal carcinoma cell extract." in: Molecular biology of the cell, Vol. 18, Issue 5, pp. 1543-53, (2007) (PubMed).

Details zu Nanog

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Andere Bezeichnung: NANOG

Hintergrund: NANOG: Nanog homeobox. Entrez Protein NP_079141. Nanog is a divergent homeodomain protein that directs pluripotency and differentiation of undifferentiated embryonic stem cells. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Human Nanog protein shares 52 % overall amino acid identity with the mouse protein and 85 % identity in the homeodomain. Human Nanog maps to gene locus 12p13.31, whereas mouse Nanog maps to gene loci 6 F2. Murine embryonic Nanog expression is detected in the inner cell mass of the blastocyst. High levels of human Nanog expression were detected by Northern analysis in the undifferentiated N-Tera embryonal carcinoma cell line.

Molekulargewicht: 35 kDa

Gen-ID: 79923

UniProt: Q9H9S0

Pathways: Stem Cell Maintenance