ITGA5 Antikörper

Produktnummer ABIN969221

Der Maus Monoklonal Anti-ITGA5-Antikörper wurde für FACS, ELISA und IHC validiert. Er ist geeignet, ITGA5 in Proben von Human zu detektieren. Es sind 3+ Publikationen verfügbar.

Kurzübersicht für ITGA5 Antikörper (ABIN969221)

Target: ITGA5 (Integrin, alpha 5 (ITGA5))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ITGA5 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), ELISA, Immunohistochemistry (IHC)

Klon: 10F6

Produktdetails anti-ITGA5 Antikörper

Verwendungszweck: ITGA5 Antibody

Aufreinigung: Ascitic fluid

Immunogen: Purified recombinant fragment of human ITGA5 expressed in E. Coli.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise:

ELISA: 1/10000

FCM: 1/200 - 1/400

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Referenzen für anti-ITGA5 Antikörper (ABIN969221)

Mould, Askari, Barton, Kline, McEwan, Craig, Humphries: "Integrin activation involves a conformational change in the alpha 1 helix of the beta subunit A-domain." in: The Journal of biological chemistry, Vol. 277, Issue 22, pp. 19800-5, (2002) (PubMed).

Zhao, Newman: "Integrin activation by regulated dimerization and oligomerization of platelet endothelial cell adhesion molecule (PECAM)-1 from within the cell." in: The Journal of cell biology, Vol. 152, Issue 1, pp. 65-73, (2001) (PubMed).

Wong, Wiedle, Ballestrem, Wehrle-Haller, Etteldorf, Bruckner, Engelhardt, Gisler, Imhof: "PECAM-1/CD31 trans-homophilic binding at the intercellular junctions is independent of its cytoplasmic domain; evidence for heterophilic interaction with integrin alphavbeta3 in Cis." in: Molecular biology of the cell, Vol. 11, Issue 9, pp. 3109-21, (2000) (PubMed).

Details zu ITGA5

Target: ITGA5 (Integrin, alpha 5 (ITGA5))

Andere Bezeichnung: ITGA5

Hintergrund: The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling. Integrin alpha 5 is a heterodimer that associates noncovalently with CD29/integrin beta 1 subunit to form the alpha-5-beta-1 very late antigen (VLA-5) complex. VLA-5 is a fibronectin receptor that is expressed on thymocytes, T-cells, monocytes and platelets. It is also found on very early B-cells and activated B-cells. VLA-5-mediated binding to fibronectin sends a costimulatory signal to T-cells and enhances Fc-gamma-R- and complement receptor-mediated phago-cytosis. It is also involved in monocyte migration into extracellular tissues.

Molekulargewicht: 114 kDa

Gen-ID: 3678

UniProt: P08648

Pathways: Integrin Complex