SLU7 Antikörper (AA 457-570)

Produktnummer ABIN968890

Dieses Maus Monoklonal-Antikörper erkennt spezifisch SLU7 in WB und IF. Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte, Hund und Huhn und wurde in 2+ Publikationen erwähnt.

Kurzübersicht für SLU7 Antikörper (AA 457-570) (ABIN968890)

Target: SLU7 (SLU7 Splicing Factor Homolog (SLU7))

Reaktivität: Human, Maus, Ratte, Hund, Huhn

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SLU7 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Klon: 35-hSlu7

Produktdetails anti-SLU7 Antikörper

Bindungsspezifität: AA 457-570

Kreuzreaktivität: Hund, Ratte (Rattus), Maus

Keine Kreuzreaktivität: Huhn

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human hSlu7 aa. 457-570

Isotyp: IgG2b

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen für anti-SLU7 Antikörper (ABIN968890)

Chua, Reed: "The RNA splicing factor hSlu7 is required for correct 3' splice-site choice." in: Nature, Vol. 402, Issue 6758, pp. 207-10, (2000) (PubMed).

Chua, Reed: "Human step II splicing factor hSlu7 functions in restructuring the spliceosome between the catalytic steps of splicing." in: Genes & development, Vol. 13, Issue 7, pp. 841-50, (1999) (PubMed).

Details zu SLU7

Target: SLU7 (SLU7 Splicing Factor Homolog (SLU7))

Andere Bezeichnung: hSlu7

Substanzklasse: Influenza Protein

Hintergrund: Splicing, the removal of introns from pre-mRNA, is mediated by spliceosomal complexes and occurs in two distinct catalytic steps. The first step involves cleavage of the 5' exon and the production of a lariat intermediate. In the second step, the 3' splice site is cleaved and the exons are fused with concomitant release of the intron lariat. The spliceosome contains multiple snRNPs and a number of non-snRNP splicing factors. Four yeast proteins (Prp16p, Prp17p, Prp18p, and Slu7p) function exclusively in the second catalytic step. Human homologs have been identified for Prp16p (hPrp16), Prp17p (hPrp17), Prp18p (hPrp18) and Slu7 (hSlu7). hSlu7 contains a zinc knuckle motif similar to the yeast Slu7. This domain is present in retroviral nucleocapsid proteins and in several splicing factors. hSlu7 associates with the spliceosome late in the splicing pathway during recognition of the 3' splice site. During step II of 3' splicing, hSlu7 tightly binds to exon 1 in the spliceosome and helps specify attack on the correct adenine-guanine dinucleotide, located 18 to 40 nucleotides downstream of the branch site.

Molekulargewicht: 80 kDa

Pathways: Ribonucleoprotein Complex Subunit Organization, SARS-CoV-2 Protein Interaktom