MAD2L2 Antikörper (AA 81-180)

Produktnummer ABIN968795

Der Maus Monoklonal Anti-MAD2L2-Antikörper wurde für WB und IF validiert. Er ist geeignet, MAD2L2 in Proben von Human und Hund zu detektieren. Es sind 2+ Publikationen verfügbar.

Kurzübersicht für MAD2L2 Antikörper (AA 81-180) (ABIN968795)

Target: MAD2L2 (MAD2 Mitotic Arrest Deficient-Like 2 (MAD2L2))

Reaktivität: Human, Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MAD2L2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Klon: 14-MAD2B-Rev7

Produktdetails anti-MAD2L2 Antikörper

Bindungsspezifität: AA 81-180

Kreuzreaktivität: Hund

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human MAD2B aa. 81-180

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN968586, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen für anti-MAD2L2 Antikörper (ABIN968795)

Chen, Fang: "MAD2B is an inhibitor of the anaphase-promoting complex." in: Genes & development, Vol. 15, Issue 14, pp. 1765-70, (2001) (PubMed).

Murakumo, Roth, Ishii, Rasio, Numata, Croce, Fishel: "A human REV7 homolog that interacts with the polymerase zeta catalytic subunit hREV3 and the spindle assembly checkpoint protein hMAD2." in: The Journal of biological chemistry, Vol. 275, Issue 6, pp. 4391-7, (2000) (PubMed).

Details zu MAD2L2

Target: MAD2L2 (MAD2 Mitotic Arrest Deficient-Like 2 (MAD2L2))

Andere Bezeichnung: MAD2B/Rev7

Hintergrund: Progression of the mammalian cell cycle is regulated by phosphorylation/dephosphorylation and synthesis/degradation of many key proteins. These events are of utmost importance at the checkpoints, or transition points, of the cell cycle. MAD2 (Mitotic Arrest-Deficient) is the human homolog of a yeast and Xenopus protein that is essential for spindle assembly during mitosis. Binding of affinity purified polyclonal antibodies to the MAD2 protein has been reported to prevent mitosis of HeLa cells. Furthermore, MAD2 is localized at the kinetochore of condensed chromosomes during mitosis and cells defective in the mitotic checkpoint have reduced levels of MAD2. MAD2 inhibits anaphase-promoting complex (APC) ubiquitin ligase activity,which is critical for controlling transitions in mitosis. This inhibition occurs through binding and suppression of CDC20 activation of APC. MAD2B, also known as Rev7, is a UV revertible gene that has 25% identity with MAD2 and also acts as an inhibitor of APC. Interestingly, MAD2B/Rev7 can bind MAD2 and interacts with the DNA polymerase zeta subunit Rev3. Thus, MAD2B may interact with mitotic checkpoint proteins and DNA repair proteins to regulate mitotic progression. This antibody is routinely tested by western blot analysis.
Synonyms: Mitotic Arrest Deficient-2B, Rev7

Molekulargewicht: 26 kDa