TNIK Antikörper (AA 522-644)

Produktnummer ABIN968788

Dieses Maus Monoklonal-Antikörper erkennt spezifisch TNIK in WB und IF. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte und wurde in 1 Publikation erwähnt.

Kurzübersicht für TNIK Antikörper (AA 522-644) (ABIN968788)

Target: TNIK (TRAF2 and NCK Interacting Kinase (TNIK))

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TNIK Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Klon: 53-TNIK

Produktdetails anti-TNIK Antikörper

Bindungsspezifität: AA 522-644

Kreuzreaktivität: Ratte (Rattus), Maus

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human TNIK aa. 522-644

Isotyp: IgG1

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20°C.

Referenzen für anti-TNIK Antikörper (ABIN968788)

Fu, Shen, Huang, Lasaga, Payan, Luo: "TNIK, a novel member of the germinal center kinase family that activates the c-Jun N-terminal kinase pathway and regulates the cytoskeleton." in: The Journal of biological chemistry, Vol. 274, Issue 43, pp. 30729-37, (1999) (PubMed).

Details zu TNIK

Target: TNIK (TRAF2 and NCK Interacting Kinase (TNIK))

Andere Bezeichnung: TNIK

Hintergrund: Ste20 is a S. cerevisiae Ser/Thr protein kinase that functions upstream of the MAP kinase module. Mammalian and yeast homologs of this kinase are divided into two classes based on their structure and regulation. Members of the first class (Ste20, Cla4, and p21-activated protein kinase) contain a C-terminal kinase domain, an N-terminal regulatory domain and a small GTPase Rac1/Cdc42 binding domain. Members of the second class lack the GTPase binding sites, but are similar to the former class throughout the catalytic domain. This second class includes germinal center kinase (GCK), HPK, KHS, KRS1 and 2, MST1, 2, and 3, NIK, SOK-1, and TNIK. Traf2- and NCK-interacting kinase (TNIK) is most homologous to another NCK-interacting kinase, NIK. TNIK contains an N- terminal kinase domain, and a C-terminal GCK homology (GCKH) domain. The mRNA of TNIK is expressed highest in heart, brain, and skeletal muscle. Overexpression of TNIK activates the JNK pathway, and leads to the disruption of F-actin structures and the inhibition of cell spreading. In vitro, TNIK can phosphorylate gelsolin. Thus, TNIK is a GCK family kinase that may regulate both the JNK pathway and cytoskeletal dynamics.

Molekulargewicht: 150 kDa