PPP1R12A Antikörper (AA 723-840)

Produktnummer ABIN968768

Der Maus Monoklonal Anti-PPP1R12A-Antikörper wurde für WB validiert. Er ist geeignet, PPP1R12A in Proben von Human, Maus und Ratte zu detektieren. Es sind 3+ Publikationen verfügbar.

Kurzübersicht für PPP1R12A Antikörper (AA 723-840) (ABIN968768)

Target: PPP1R12A (Myosin Phosphatase, Target Subunit 1 (PPP1R12A))

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PPP1R12A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Klon: 20-MYPT1

Produktdetails anti-PPP1R12A Antikörper

Bindungsspezifität: AA 723-840

Kreuzreaktivität: Human, Maus

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Rat MYPT1 aa. 723-840

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN968545, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20°C.

Referenzen für anti-PPP1R12A Antikörper (ABIN968768)

Khatri, Joyce, Brozovich, Fisher: "Role of myosin phosphatase isoforms in cGMP-mediated smooth muscle relaxation." in: The Journal of biological chemistry, Vol. 276, Issue 40, pp. 37250-7, (2001) (PubMed).

Kimura, Ito, Amano, Chihara, Fukata, Nakafuku, Yamamori, Feng, Nakano, Okawa, Iwamatsu, Kaibuchi: "Regulation of myosin phosphatase by Rho and Rho-associated kinase (Rho-kinase)" in: Science (New York, N.Y.), Vol. 273, Issue 5272, pp. 245-8, (1996) (PubMed).

Chen, Chen, Alessi, Campbell, Shanahan, Cohen, Cohen: "Molecular cloning of cDNA encoding the 110 kDa and 21 kDa regulatory subunits of smooth muscle protein phosphatase 1M." in: FEBS letters, Vol. 356, Issue 1, pp. 51-5, (1995) (PubMed).

Details zu PPP1R12A

Target: PPP1R12A (Myosin Phosphatase, Target Subunit 1 (PPP1R12A))

Andere Bezeichnung: MYPT1

Hintergrund: Protein phosphatase-1 (PP1) is a major serine/threonine phosphatase that is involved in various cellular processes in eukaryotic cells. The PP1 catalytic subunit may be directed to particular subcellular locations by targeting subunits, which can modify the substrate specificity of the enzyme. The PP1 enzyme associated with the myofibrils of striated and smooth muscle forms a complex with Myosin Phosphatase Targeting subunit 1 (MYPT1). MYPT1 contains seven ankyrin repeats in the N-terminal region, and a leucine zipper (LZ) motif in the C-terminus. Alternative splicing of the MYPT1 mRNA leads to expression of a truncated MYPT1 that lacks the leucine zipper region. The expression of MYPT1 splice variants correlate with a smooth muscle phenotype. In the tonic contracting chicken aorta, only the full length MYPT1 is expressed, while in the phasic contracting chicken gizzard, only the truncated MYPT1 is expressed. The leucine zipper motif may be required for association with cGMP-dependent protein kinase I. Thus, MYPT1 may direct not only PP1 enzymatic activity toward myosin, but also may regulate the cGMP responsiveness of PP1 through expression of alternative splice variants.
Synonyms: Myosin Phosphatase Targeting subunit 1

Molekulargewicht: 130 kDa

Pathways: M Phase