DLC1 Antikörper (AA 47-249)

Produktnummer ABIN968714

Der Maus Monoklonal Anti-DLC1-Antikörper wurde für WB und IF validiert. Er ist geeignet, DLC1 in Proben von Human zu detektieren. Es sind 3+ Publikationen verfügbar.

Kurzübersicht für DLC1 Antikörper (AA 47-249) (ABIN968714)

Target: DLC1 (Deleted in Liver Cancer 1 (DLC1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser DLC1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Klon: 3-DLC

Produktdetails anti-DLC1 Antikörper

Bindungsspezifität: AA 47-249

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Please refer to us for technical protocols.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human DLC-1 aa. 47-249

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen für anti-DLC1 Antikörper (ABIN968714)

Sekimata, Kabuyama, Emori, Homma: "Morphological changes and detachment of adherent cells induced by p122, a GTPase-activating protein for Rho." in: The Journal of biological chemistry, Vol. 274, Issue 25, pp. 17757-62, (1999) (PubMed).

Yuan, Miller, Keck, Zimonjic, Thorgeirsson, Popescu: "Cloning, characterization, and chromosomal localization of a gene frequently deleted in human liver cancer (DLC-1) homologous to rat RhoGAP." in: Cancer research, Vol. 58, Issue 10, pp. 2196-9, (1998) (PubMed).

Homma, Emori: "A dual functional signal mediator showing RhoGAP and phospholipase C-delta stimulating activities." in: The EMBO journal, Vol. 14, Issue 2, pp. 286-91, (1995) (PubMed).

Details zu DLC1

Target: DLC1 (Deleted in Liver Cancer 1 (DLC1))

Andere Bezeichnung: DLC-1

Hintergrund: Members of the Ras superfamily of GTPases are distantly related to the heterotrimeric G proteins and shuttle between inactive and active GTP-bound forms. Conversion between these forms is controlled by guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs). Rho is a GTPase that regulates cell morphology and motility in response to extracellular signals. p190A, p190B, and p122 RhoGAPs all contain RhoGAP domains and regulate the GTPase activity of Rho. p122 RhoGAP exhibits GAP activity for RhoA, but not Rac1, and binds and activates PLC-delta1. Microinjection of p122 RhoGAP suppresses LPA-induced formation of stress fibers and focal adhesions. Deleted in liver cancer-1 (DLC-1) was identified by its frequent deletion in liver cancer and is a human homologue of rat p122 RhoGAP. DLC-1 is expressed in many hepatocellular carcinoma tissues and cell lines and could be an important tumor suppressor gene. In normal human tissues, DLC-1 is expressed widely with the highest expression in heart and lung. Both p122 RhoGAP and DLC-1 have RhoGAP domains toward their C-terminal regions, which is thought to be important for regulation of Rho GTPase activity in many tissues. This antibody is routinely tested by western blot analysis.

Molekulargewicht: 123 kDa

Pathways: Tube Formation, Positive Regulation of Endopeptidase Activity