Cullin 3 Antikörper (AA 565-684)

Produktnummer ABIN968679

Dieses Anti-Cullin 3-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Cullin 3 in WB und IF. Geeignet für Human, Maus, Ratte, Huhn und Hund. Dieses Primary Antibody wurde in 2+ Publikationen zitiert.

Kurzübersicht für Cullin 3 Antikörper (AA 565-684) (ABIN968679)

Target: Cullin 3 (CUL3)

Reaktivität: Human, Maus, Ratte, Huhn, Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Cullin 3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Klon: 3-CUL

Produktdetails anti-Cullin 3 Antikörper

Bindungsspezifität: AA 565-684

Kreuzreaktivität: Maus, Ratte (Rattus), Hund, Huhn

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human CUL-3 aa. 565-684

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN968545, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen für anti-Cullin 3 Antikörper (ABIN968679)

Hori, Osaka, Chiba, Miyamoto, Okabayashi, Shimbara, Kato, Tanaka: "Covalent modification of all members of human cullin family proteins by NEDD8." in: Oncogene, Vol. 18, Issue 48, pp. 6829-34, (2000) (PubMed).

Singer, Gurian-West, Clurman, Roberts: "Cullin-3 targets cyclin E for ubiquitination and controls S phase in mammalian cells." in: Genes & development, Vol. 13, Issue 18, pp. 2375-87, (1999) (PubMed).

Details zu Cullin 3

Target: Cullin 3 (CUL3)

Andere Bezeichnung: CUL-3

Hintergrund: The cullins are a family of proteins that are integral to cell cycle regulation. Members of this family include human Cul proteins, C.elegans Ce-Cul-1, and S. cerevisiae Cdc53. Proper control of cell cycle progression is essential for the prevention of tumorigenesis. Mutant forms of both Ce-Cul-1 and Cdc53 have been implicated in the oncogenic process. The human Cul family of proteins, Cul-1, -2, -3, -4A, -4B, and -5, have also been implicated in oncogenic processes. Abnormal nuclear localization of Cul-2 is seen in a rare hereditary condition known as VHL (von Hippel-Lindau) syndrome. Patients with VHL syndrome possess a mutant VHL gene and develop cancers such as retinal anginomas, CNS hemangioblastomas, and renal cell carcinomas. Cul-3 is widely expressed in normal cells and tissues, and shows increased expression in colon cancer cells. Cul-3 can associate with cyclin E in mammalian cells, and overexpression of Cul-3 targets cyclin E for ubiquination, while deletion of Cul-3 in mice leads to increased cyclin E accumulation and abnormal regulation of S-phase. Thus, the Cul family of proteins may have roles in cell cycle regulation and tumoriogenesis. This antibody is routinely tested by western blot analysis.

Molekulargewicht: 89 kDa

Pathways: M Phase