ARHGEF7 Antikörper (AA 351-453)

Produktnummer ABIN968623

Dieses Maus Monoklonal-Antikörper erkennt spezifisch ARHGEF7 in WB und IF. Er zeigt eine Reaktivität gegenüber Ratte, Maus, Huhn und Hund und wurde in 4+ Publikationen erwähnt.

Kurzübersicht für ARHGEF7 Antikörper (AA 351-453) (ABIN968623)

Target: ARHGEF7 (rho Guanine Nucleotide Exchange Factor (GEF) 7 (ARHGEF7))

Reaktivität: Ratte, Maus, Huhn, Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ARHGEF7 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Klon: 23-bPIX

Produktdetails anti-ARHGEF7 Antikörper

Bindungsspezifität: AA 351-453

Kreuzreaktivität: Huhn, Hund, Maus

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Rat betaPIX aa. 351-453

Isotyp: IgG1

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20°C.

Referenzen für anti-ARHGEF7 Antikörper (ABIN968623)

Turner, Brown, Perrotta, Riedy, Nikolopoulos, McDonald, Bagrodia, Thomas, Leventhal: "Paxillin LD4 motif binds PAK and PIX through a novel 95-kD ankyrin repeat, ARF-GAP protein: A role in cytoskeletal remodeling." in: The Journal of cell biology, Vol. 145, Issue 4, pp. 851-63, (1999) (PubMed).

Bagrodia, Taylor, Jordon, Van Aelst, Cerione: "A novel regulator of p21-activated kinases." in: The Journal of biological chemistry, Vol. 273, Issue 37, pp. 23633-6, (1998) (PubMed).

Manser, Loo, Koh, Zhao, Chen, Tan, Tan, Leung, Lim: "PAK kinases are directly coupled to the PIX family of nucleotide exchange factors." in: Molecular cell, Vol. 1, Issue 2, pp. 183-92, (1998) (PubMed).

Oh, Yoo, Jo, Song, Kim, Park: "Cloning of a SH3 domain-containing proline-rich protein, p85SPR, and its localization in focal adhesion." in: Biochemical and biophysical research communications, Vol. 235, Issue 3, pp. 794-8, (1997) (PubMed).

Details zu ARHGEF7

Target: ARHGEF7 (rho Guanine Nucleotide Exchange Factor (GEF) 7 (ARHGEF7))

Andere Bezeichnung: beta PIX

Hintergrund: The activity of PAK family kinases is regulated through interaction with the small GTPases Cdc42 and Rac1. PAKs are activated by the GTP-bound form of Cdc-42 and Rac1, and recruitment of PAKs to focal complexes has been implicated in Cdc42- and Rac1-dependent regulation of focal contact formation. PAK-interacting exchange factor (PIX) was identified in a screen for proteins that bind PAKs. Two forms of PIX have been identified: an 85 kDa protein designated alphaPIX and a 78 kDa protein designated betaPIX. These proteins have 80% identity in their overlapping regions, which include myosin-like, pleckstrin (PH), Dbl (DH), and SH3 domains. In addition, alphaPIX contains a calponin-like domain at the N-terminus. The expression of betaPIX is ubiquitous, while alphaPIX is expressed in heart, muscle, and thymus. PIX can act as a guanine nuleotide exchange factor for Rac1 and co-transfection of betaPIX, Cdc42, and alphaPAK results in increased alphaPAK activity. PIX binding to PAK is required for localization of PAKs to focal complexes and injection of betaPIX leads to rac1-dependent membrane ruffling. Thus, PIX is important for PAK localization and activity during small GTPase-dependent regulation of cell morphology.

Molekulargewicht: 78 kDa

Pathways: EGFR Signaling Pathway, Neurotrophin Signalübertragung, EGFR Downregulation