APAF1 Antikörper (AA 252-445)

Produktnummer ABIN968496

Dieses Anti-APAF1-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von APAF1 in WB und IF. Geeignet für Human. Dieses Primary Antibody wurde in 5+ Publikationen zitiert.

Kurzübersicht für APAF1 Antikörper (AA 252-445) (ABIN968496)

Target: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser APAF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Klon: 24-Apaf

Produktdetails anti-APAF1 Antikörper

Bindungsspezifität: AA 252-445

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Apaf-1 aa. 252-445

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN968536, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen für anti-APAF1 Antikörper (ABIN968496)

Li, Trudel, Wogan: "Nitric oxide-induced genotoxicity, mitochondrial damage, and apoptosis in human lymphoblastoid cells expressing wild-type and mutant p53." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 16, pp. 10364-9, (2002) (PubMed).

Cain, Brown, Langlais, Cohen: "Caspase activation involves the formation of the aposome, a large (approximately 700 kDa) caspase-activating complex." in: The Journal of biological chemistry, Vol. 274, Issue 32, pp. 22686-92, (1999) (PubMed).

Hu, Benedict, Ding, Núñez: "Role of cytochrome c and dATP/ATP hydrolysis in Apaf-1-mediated caspase-9 activation and apoptosis." in: The EMBO journal, Vol. 18, Issue 13, pp. 3586-95, (1999) (PubMed).

Soengas, Alarcón, Yoshida, Giaccia, Hakem, Mak, Lowe: "Apaf-1 and caspase-9 in p53-dependent apoptosis and tumor inhibition." in: Science (New York, N.Y.), Vol. 284, Issue 5411, pp. 156-9, (1999) (PubMed).

Slee, Harte, Kluck, Wolf, Casiano, Newmeyer, Wang, Reed, Nicholson, Alnemri, Green, Martin: "Ordering the cytochrome c-initiated caspase cascade: hierarchical activation of caspases-2, -3, -6, -7, -8, and -10 in a caspase-9-dependent manner." in: The Journal of cell biology, Vol. 144, Issue 2, pp. 281-92, (1999) (PubMed).

Details zu APAF1

Target: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Andere Bezeichnung: Apaf-1

Hintergrund: The process of apoptosis requires the activation of aspartate-specific cystenine proteases in the caspase family. Group I caspases (1,4,5) cleave at (W/L)EHD tetrapeptide motifs, while group II caspases (2,3,7) cleave the DEXD tetrapeptide motif. Group III caspases (6,8,9) are activators of other caspases via cleavage of (I/V)EXD tetrapeptide sequences. Apoptotic protease-activating factor-1 (Apaf-1), cytochrome c, and dATP activate caspase-9, which in turn, initiates the post-mitochondrial-mediated caspase cascade that includes caspase-2, 3, 6, 7, 8 and 10. Apaf-1 is a soluble protein with a short N-terminal caspase recruitment domain (CARD), a central CED-4 homology domain, and 12 WD-40 repeats that may be involved in protein-protein interactions. During apoptosis, a large (700 kDa) aposome complex containing Apaf-1, cytochrome c, caspase-3, 7, and 9, and a smaller (200-300 kDa) microaposome complex containing caspase-3 and 7 exhibit higher cleavage activity than free caspase heterotetramers. Thus Apaf-1 is a component of the large aposome complex, which functions in caspase activation leading to caspase-dependent proteolytic events and apoptosis. This antibody is routinely tested by western blot analysis.
Synonyms: Apoptotic Protease Activating Factor-1

Molekulargewicht: 130 kDa

Pathways: p53 Signalweg, Apoptose, Caspase Kaskade in der Apoptose, Tube Formation, Positive Regulation of Endopeptidase Activity