BIRC6 Antikörper (AA 372-571)

Produktnummer ABIN968409

Dieses Anti-BIRC6-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von BIRC6 in WB und IF. Geeignet für Human, Maus, Hund und Ratte. Dieses Primary Antibody wurde in 1 Publikation zitiert.

Kurzübersicht für BIRC6 Antikörper (AA 372-571) (ABIN968409)

Target: BIRC6 (Baculoviral IAP Repeat-Containing 6 (BIRC6))

Reaktivität: Human, Maus, Hund, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser BIRC6 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Klon: 4-BRUCE

Produktdetails anti-BIRC6 Antikörper

Bindungsspezifität: AA 372-571

Kreuzreaktivität: Human, Ratte (Rattus), Hund

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse BRUCE aa. 372-571

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Clone 4/BRUCE has also been shown to work well for Western blot application on Hela lysate (ABIN968535)

Kommentare:

Related Products: ABIN968552, ABIN967389, ABIN968535

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20°C.

Referenzen für anti-BIRC6 Antikörper (ABIN968409)

Hauser, Bardroff, Pyrowolakis, Jentsch: "A giant ubiquitin-conjugating enzyme related to IAP apoptosis inhibitors." in: The Journal of cell biology, Vol. 141, Issue 6, pp. 1415-22, (1998) (PubMed).

Details zu BIRC6

Target: BIRC6 (Baculoviral IAP Repeat-Containing 6 (BIRC6))

Andere Bezeichnung: BRUCE

Hintergrund: Selective proteolysis is essential for the modulation of key cellular processes such as cell cycle progression. However, unlike other post-translational events, proteolysis is irreversible and therefore must occur in unidirectional cellular pathways. In eukaryotes, proteolysis is mediated primarily by the ubiquitin pathway. This pathway designates proteins for degradation by the proteasome, a multicatalytic protease complex. The ubiquitin pathway is a multistep system that tags proteins for degradation via the attachment of ubiquitin molecules to the target. This attachment is mediated by the ubiquitin activating/conjugating enzymes E1, E2, E3, and BRUCE. BRUCE (BIR Repeat containing Ubiquitin-Conjugating Enzyme) is a novel enzyme that associates with the Golgi and the vesicular system. It contains a UBC (ubiquitin conjugating enzyme) domain, which is essential for catalysis, and a BIR (baculovirus inhibitor of apoptosis repeat) motif. BIR motifs are also found within inhibitor of apoptosis proteins (IAP) and are critical for anti-apoptotic activity. Therefore, BRUCE may function to both mediate ubiquitin-dependent proteolysis and contribute to anti-apoptotic cellular pathways.
Synonyms: BIR Repeat containing Ubiquitin-Conjugating Enzyme

Molekulargewicht: 528 kDa