Dynamitin Antikörper (AA 55-196)

Produktnummer ABIN968308

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Dynamitin in WB und IF. Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte und Blow Fly und wurde in 5+ Publikationen erwähnt.

Kurzübersicht für Dynamitin Antikörper (AA 55-196) (ABIN968308)

Target: Dynamitin (DCTN2) (Dynactin 2 (p50) (DCTN2))

Reaktivität: Human, Maus, Ratte, Blow Fly

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Dynamitin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Klon: 25-Dynactin p50

Produktdetails anti-Dynamitin Antikörper

Bindungsspezifität: AA 55-196

Kreuzreaktivität: Ratte (Rattus), Maus, Insekten

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Dynactin p50 aa. 55-196

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN968537, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen für anti-Dynamitin Antikörper (ABIN968308)

Kuo, Liu, Wei, Lin-Lee, Tatebe, Mills, Unate: "Induction of human MDR1 gene expression by 2-acetylaminofluorene is mediated by effectors of the phosphoinositide 3-kinase pathway that activate NF-kappaB signaling." in: Oncogene, Vol. 21, Issue 13, pp. 1945-54, (2002) (PubMed).

Holleran, Ligon, Tokito, Stankewich, Morrow, Holzbaur: "beta III spectrin binds to the Arp1 subunit of dynactin." in: The Journal of biological chemistry, Vol. 276, Issue 39, pp. 36598-605, (2001) (PubMed).

Starr, Williams, Hays, Goldberg: "ZW10 helps recruit dynactin and dynein to the kinetochore." in: The Journal of cell biology, Vol. 142, Issue 3, pp. 763-74, (1998) (PubMed).

Burkhardt, Echeverri, Nilsson, Vallee: "Overexpression of the dynamitin (p50) subunit of the dynactin complex disrupts dynein-dependent maintenance of membrane organelle distribution." in: The Journal of cell biology, Vol. 139, Issue 2, pp. 469-84, (1997) (PubMed).

Echeverri, Paschal, Vaughan, Vallee: "Molecular characterization of the 50-kD subunit of dynactin reveals function for the complex in chromosome alignment and spindle organization during mitosis." in: The Journal of cell biology, Vol. 132, Issue 4, pp. 617-33, (1996) (PubMed).

Details zu Dynamitin

Target: Dynamitin (DCTN2) (Dynactin 2 (p50) (DCTN2))

Andere Bezeichnung: Dynactin p50

Hintergrund: Dyneins are ubiquitous, multimeric proteins that are responsible for minus end-directed microtubule-based organelle transport. They are divided into two classes, cytosolic and axonemal. The activity of cytosolic dynein is regulated by the multiprotein dynactin complex with which it co-purifies. Dynactin is composed of at least nine polypeptides with p50 being the second most abundant subunit. The complex consists of an F-actin-like core filament of actin-related protein Arp1, a heterodimer that acts as a cap at one end, and a 62kDa subunit at the other end. p150[glued] is the largest subunit of the complex. The precise function of dynactin remains to be determined, but it may be recruited to prometaphase kinetochores to assist in alignment and spindle organization during mitosis. Additionally, dynactin localizes at the tail of cytosolic dynein and activates dynein-mediated vesicle movement. Therefore, dynactin may function to anchor or target dynein to the kinetochore during mitosis.

Molekulargewicht: 50 kDa

Pathways: M Phase, Ribonucleoprotein Complex Subunit Organization