ARF3 Antikörper (AA 1-181)

Produktnummer ABIN968175

Dieses Anti-ARF3-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von ARF3 in WB, IHC, IF und IP. Geeignet für Human, Maus, Ratte, Huhn, Hund und Frosch. Dieses Primary Antibody wurde in 5+ Publikationen zitiert.

Kurzübersicht für ARF3 Antikörper (AA 1-181) (ABIN968175)

Target: ARF3 (ADP-Ribosylation Factor 3 (ARF3))

Reaktivität: Human, Maus, Ratte, Huhn, Hund, Frosch

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ARF3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Klon: 41-ARF3

Produktdetails anti-ARF3 Antikörper

Bindungsspezifität: AA 1-181

Kreuzreaktivität: Ratte (Rattus), Maus, Huhn, Hund, Frosch

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human ARF3 aa. 1-181

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN968545, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen für anti-ARF3 Antikörper (ABIN968175)

Lee, Stevens, Hsu, Tsai, Lee, Moss, Vaughan: "Expression in human endothelial cells of ADP-ribosylation factors, 20-kDa guanine nucleotide-binding proteins involved in the initiation of vesicular transport." in: Journal of molecular and cellular cardiology, Vol. 28, Issue 9, pp. 1911-20, (1997) (PubMed).

Morinaga, Tsai, Moss, Vaughan: "Isolation of a brefeldin A-inhibited guanine nucleotide-exchange protein for ADP ribosylation factor (ARF) 1 and ARF3 that contains a Sec7-like domain." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 23, pp. 12856-60, (1996) (PubMed).

Cockcroft, Thomas, Fensome, Geny, Cunningham, Gout, Hiles, Totty, Truong, Hsuan: "Phospholipase D: a downstream effector of ARF in granulocytes." in: Science (New York, N.Y.), Vol. 263, Issue 5146, pp. 523-6, (1994) (PubMed).

Moss, Vaughan: "ADP-ribosylation factors, 20,000 M(r) guanine nucleotide-binding protein activators of cholera toxin and components of intracellular vesicular transport systems." in: Cellular signalling, Vol. 5, Issue 4, pp. 367-79, (1993) (PubMed).

Kahn, Radding: "Separation of the presynaptic and synaptic phases of homologous pairing promoted by recA protein." in: The Journal of biological chemistry, Vol. 259, Issue 12, pp. 7495-503, (1984) (PubMed).

Details zu ARF3

Target: ARF3 (ADP-Ribosylation Factor 3 (ARF3))

Andere Bezeichnung: ARF3

Hintergrund: The ADP-ribosylation factors (ARFs) belong to the multigene family of small GTPases capable of activating cholera toxin. ARFs fall into three different classes: Class I is composed of ARF-1, ARF-2, and ARF-3, Class II consists of ARF-4 and ARF-5, and Class III includes ARF6. Unique to ARFs is their lack of intrinsic GTP hydrolysis activity, a high affinity for GDP in a Mg2+-dependent manner, and phospholipid requirement for nucleotide exchange. ARFs are involved in intravesicular acidification of microsomal vesicles, endosome fusion, nuclear membrane assembly, and formation of clathrin-coated vesicles. In addition, GTP and ARF-3 are required for the activation of phospholipase D (PLD), an early cellular response triggered by the binding of receptors on the cell surface in response to numerous extracellular signals. Although predominantly cytosolic, ARF-3 can be translocated to cellular membranes upon cellular stimulation. The transition between the GDP-bound ARF-3 to the GTP-ARF-3 is facilitated by a high molecular weight guanine nucleotide-exchange factor sensitive to brefeldin. ARF-3 has been reported to be the most abundant in brain, kidney, and liver.
Synonyms: ADP Ribosylation Factor-3

Molekulargewicht: 20 kDa

Pathways: Inositol Metabolic Process, Cellular Glucan Metabolic Process