UBE2I Antikörper (AA 26-156)

Produktnummer ABIN968155

Dieses Maus Monoklonal-Antikörper erkennt spezifisch UBE2I in WB, IHC, IF und IP. Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte, Huhn und Hund und wurde in 4+ Publikationen erwähnt.

Kurzübersicht für UBE2I Antikörper (AA 26-156) (ABIN968155)

Target: UBE2I (Ubiquitin-Conjugating Enzyme E2I (UBE2I))

Reaktivität: Human, Maus, Ratte, Huhn, Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser UBE2I Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Klon: 50-Ubc9

Produktdetails anti-UBE2I Antikörper

Bindungsspezifität: AA 26-156

Kreuzreaktivität: Hund, Ratte (Rattus), Maus, Huhn

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Ubc9 aa. 26-156

Isotyp: IgG2a

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen für anti-UBE2I Antikörper (ABIN968155)

Saitoh, Pizzi, Wang: "Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358." in: The Journal of biological chemistry, Vol. 277, Issue 7, pp. 4755-63, (2002) (PubMed).

Buschmann, Lerner, Lee, Ronai: "The Mdm-2 amino terminus is required for Mdm2 binding and SUMO-1 conjugation by the E2 SUMO-1 conjugating enzyme Ubc9." in: The Journal of biological chemistry, Vol. 276, Issue 44, pp. 40389-95, (2001) (PubMed).

Kovalenko, Plug, Haaf, Gonda, Ashley, Ward, Radding, Golub: "Mammalian ubiquitin-conjugating enzyme Ubc9 interacts with Rad51 recombination protein and localizes in synaptonemal complexes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 7, pp. 2958-63, (1996) (PubMed).

Wang, Qiu, Seufert, Taguchi, Testa, Whitmore, Callen, Welsh, Shenk, Deuel: "Molecular cloning of the cDNA and chromosome localization of the gene for human ubiquitin-conjugating enzyme 9." in: The Journal of biological chemistry, Vol. 271, Issue 40, pp. 24811-6, (1996) (PubMed).

Details zu UBE2I

Target: UBE2I (Ubiquitin-Conjugating Enzyme E2I (UBE2I))

Andere Bezeichnung: Ubc9

Hintergrund: Progression of the mammalian cell cycle is primarily regulated by phosphorylation/dephosphorylation and synthesis/degradation of many key proteins. Ubiquitin, a soluble protein of 76 amino acids, is enzymatically attached to an e-NH2-Lys in a target protein. Ubiquitination is a hallmark for rapid protein degradation of the target protein in the proteosome (a cytoplasmic complex of proteases). Human homologs of the yeast ubiquitin-conjugating enzymes (Ubc) have been reported, including Ubc9. Ubc9 is 158 amino acids with an apparent molecular weight of 18kDa. Although ubiquitously expressed, the highest levels of Ubc9 are found in testis and thymus. Ubc9 was localized to the synaptonemal complex in male mouse sex chromosomes. Furthermore, Ubc9 interacts with the recombination protein Rad51, thus suggesting an important role for Ubc9 during meiosis.

Molekulargewicht: 18 kDa

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Ubiquitin Proteasome Pathway