PKN1 Antikörper (AA 215-388)

Produktnummer ABIN968110

Dieses Maus Monoklonal-Antikörper erkennt spezifisch PKN1 in WB, IHC, IF und IP. Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte und Hund und wurde in 4+ Publikationen erwähnt.

Kurzübersicht für PKN1 Antikörper (AA 215-388) (ABIN968110)

Target: PKN1 (Protein Kinase N1 (PKN1))

Reaktivität: Human, Maus, Ratte, Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PKN1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Klon: 49-PRK1

Produktdetails anti-PKN1 Antikörper

Bindungsspezifität: AA 215-388

Kreuzreaktivität: Hund, Ratte (Rattus), Maus

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human PRK1 aa. 215-388

Isotyp: IgG1

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20°C.

Referenzen für anti-PKN1 Antikörper (ABIN968110)

Hughes, Larijani, Parker: "Detecting protein-phospholipid interactions. Epidermal growth factor-induced activation of phospholipase D1b in situ." in: The Journal of biological chemistry, Vol. 277, Issue 25, pp. 22974-9, (2002) (PubMed).

Flynn, Mellor, Casamassima, Parker: "Rho GTPase control of protein kinase C-related protein kinase activation by 3-phosphoinositide-dependent protein kinase." in: The Journal of biological chemistry, Vol. 275, Issue 15, pp. 11064-70, (2000) (PubMed).

Palmer, Ridden, Parker: "Cloning and expression patterns of two members of a novel protein-kinase-C-related kinase family." in: European journal of biochemistry / FEBS, Vol. 227, Issue 1-2, pp. 344-51, (1995) (PubMed).

Mukai, Kitagawa, Shibata, Takanaga, Mori, Shimakawa, Miyahara, Hirao, Ono: "Activation of PKN, a novel 120-kDa protein kinase with leucine zipper-like sequences, by unsaturated fatty acids and by limited proteolysis." in: Biochemical and biophysical research communications, Vol. 204, Issue 1, pp. 348-56, (1994) (PubMed).

Details zu PKN1

Target: PKN1 (Protein Kinase N1 (PKN1))

Andere Bezeichnung: PRK1

Hintergrund: Members of the Protein Kinase C (PKC) family of homologous serine/threonine protein kinases are involved in a number of processes such as cell growth, cell differentiation, and cytokine secretion. PKCs are activated by Ca2+, phospholipids, diacylglycerol, phorbol esters, and proteolysis. PRK1 (PKC-Related Kinase 1, also named PKN) was originally identified in human hippocampus as a novel protein kinase with sequence homology to PKC. PRK1 contains 942 amino acids with an apparent molecular weight of 120 kDa. Although activated by limited proteolysis, PRK1 is not activated by Ca2+/diacylglycerol or phorbol esters. However, PRK1 is activated by phospholipids and arachidonic acid. PRK1 may regulate cytoskeletal changes since it binds to Rho-GTP and becomes phosphorylated in vivo, coincidentally with the formation of focal adhesions and stress fibers.
Synonyms: PKN

Molekulargewicht: 120 kDa