NEK2 Antikörper (AA 244-444)

Produktnummer ABIN968054

Der Maus Monoklonal Anti-NEK2-Antikörper wurde für WB, IP, IF und IHC (f) validiert. Er ist geeignet, NEK2 in Proben von Human zu detektieren. Es sind 2+ Publikationen verfügbar.

Kurzübersicht für NEK2 Antikörper (AA 244-444) (ABIN968054)

Target: NEK2 (NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser NEK2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: 20-Nek2

Produktdetails anti-NEK2 Antikörper

Bindungsspezifität: AA 244-444

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Nek2 aa. 244-444

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN968537, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen für anti-NEK2 Antikörper (ABIN968054)

Fry, Schultz, Bartek, Nigg: "Substrate specificity and cell cycle regulation of the Nek2 protein kinase, a potential human homolog of the mitotic regulator NIMA of Aspergillus nidulans." in: The Journal of biological chemistry, Vol. 270, Issue 21, pp. 12899-905, (1995) (PubMed).

Schultz, Fry, Sütterlin, Ried, Nigg: "Cell cycle-dependent expression of Nek2, a novel human protein kinase related to the NIMA mitotic regulator of Aspergillus nidulans." in: Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research, Vol. 5, Issue 6, pp. 625-35, (1994) (PubMed).

Details zu NEK2

Target: NEK2 (NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2))

Andere Bezeichnung: Nek2

Hintergrund: Reversible protein phosphorylation is critical for progression through the cell cycle and mitosis. In Aspergillus nidulans, the nima gene (never in mitosis) encodes a protein kinase that is essential for mitosis. Three human genes (nek1, 2, and 3 [Nima-related kinase]) with significant homology to the A. nidulans nima have been reported. The nek2 gene encodes a protein of 445 amino acids and, like its fungal homolog, its expression is regulated throughout the cell cycle. In Hela cells, Nek2 activity and expression are low during M and G1 phases of the cell cycle. However, both parameters increase during S phase and mitosis. In addition, Nek2 phosphorylates protein substrates exclusively at serine and threonine residues. Thus, like its fungal homolog, Nek2 may be a crucial element in controlling the cell's entry into S phase and mitosis. This antibody is routinely tested by western blot analysis.
Synonyms: Nima Related Kinase 2

Molekulargewicht: 46 kDa

Pathways: M Phase