Synaptotagmin Antikörper (AA 72-223)

Produktnummer ABIN967965

Dieses Anti-Synaptotagmin-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Synaptotagmin in WB. Geeignet für Ratte. Dieses Primary Antibody wurde in 5+ Publikationen zitiert.

Kurzübersicht für Synaptotagmin Antikörper (AA 72-223) (ABIN967965)

Target: Synaptotagmin (SYT)

Reaktivität: Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Synaptotagmin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Klon: BC17

Produktdetails anti-Synaptotagmin Antikörper

Bindungsspezifität: AA 72-223

Kreuzreaktivität: Human

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: Purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Rat Synaptotagmin

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN968545, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 µg/ml

Buffer: Aqueous buffered solution containing BSA, glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Referenzen für anti-Synaptotagmin Antikörper (ABIN967965)

Liu, Fallon, Lashuel, Liu, Lansbury: "The UCH-L1 gene encodes two opposing enzymatic activities that affect alpha-synuclein degradation and Parkinson's disease susceptibility." in: Cell, Vol. 111, Issue 2, pp. 209-18, (2002) (PubMed).

Ramalho-Santos, Moreno, Sutovsky, Chan, Hewitson, Wessel, Simerly, Schatten: "SNAREs in mammalian sperm: possible implications for fertilization." in: Developmental biology, Vol. 223, Issue 1, pp. 54-69, (2000) (PubMed).

Duncan, Don-Wauchope, Tapechum, Shipston, Chow, Estibeiro: "High-efficiency Semliki Forest virus-mediated transduction in bovine adrenal chromaffin cells." in: The Biochemical journal, Vol. 342 Pt 3, pp. 497-501, (1999) (PubMed).

Scheller: "Membrane trafficking in the presynaptic nerve terminal." in: Neuron, Vol. 14, Issue 5, pp. 893-7, (1995) (PubMed).

Perin, Johnston, Ozcelik, Jahn, Francke, Südhof: "Structural and functional conservation of synaptotagmin (p65) in Drosophila and humans." in: The Journal of biological chemistry, Vol. 266, Issue 1, pp. 615-22, (1991) (PubMed).

Details zu Synaptotagmin

Target: Synaptotagmin (SYT)

Andere Bezeichnung: Synaptotagmin

Hintergrund: Synaptotagmin (p65) is an abundant synaptic vesicle protein that contains a single transmembrane region and two copies of an internal repeat that is homologous to the regulatory region of Protein Kinase C. It appears that synaptotagmin has a regulatory role in the synaptic vesicle pathway, particularly in vesicle docking and/or fusion with the plasmalemma. A model has been proposed to explain docking, activation, and fusion of synaptic vesicles with donor membranes. This model suggests that VAMP/synaptobrevin and synaptotagmin (vSNARE) on the synaptic vesicle, and SNAP-25 and syntaxin (tSNAREs) on the plasma membrane, interact to form a 7S complex. Two additional soluble proteins, alphaSNAP and NSF, are later added to the 7S complex, accompanied by the loss of synaptotagmin. The resulting 20S complex contains syntaxin, SNAP-25, VAMP, alphaSNAP, and NSF. Genetic studies in several species demonstrate that mutation or deletion of synaptotagmin results in a large decrease in Ca2+ triggered transmitter release. Mammalian synapses that lack synaptotagmin show a selective decrease in a fast component of release, suggesting that synaptotagmin is the Ca2+ sensor triggering exocytosis.

Molekulargewicht: 65 kDa