Villin 1 Antikörper (AA 1-827)

Produktnummer ABIN967920

Dieses Anti-Villin 1-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Villin 1 in IHC, WB, IF und IP. Geeignet für Human. Dieses Primary Antibody wurde in 5+ Publikationen zitiert.

Kurzübersicht für Villin 1 Antikörper (AA 1-827) (ABIN967920)

Target: Villin 1 (VIL1)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Villin 1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Klon: 12-Villin

Produktdetails anti-Villin 1 Antikörper

Bindungsspezifität: AA 1-827

Kreuzreaktivität: Human

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Cow Villin aa. 1-827

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN968551, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen für anti-Villin 1 Antikörper (ABIN967920)

Nies, König, Cui, Brom, Spring, Keppler: "Structural requirements for the apical sorting of human multidrug resistance protein 2 (ABCC2)." in: European journal of biochemistry / FEBS, Vol. 269, Issue 7, pp. 1866-76, (2002) (PubMed).

Zhai, Zhao, Panebra, Guerrerio, Khurana: "Tyrosine phosphorylation of villin regulates the organization of the actin cytoskeleton." in: The Journal of biological chemistry, Vol. 276, Issue 39, pp. 36163-7, (2001) (PubMed).

McSwine, Musch, Bookstein, Xie, Rao, Chang: "Regulation of apical membrane Na+/H+ exchangers NHE2 and NHE3 in intestinal epithelial cell line C2/bbe." in: The American journal of physiology, Vol. 275, Issue 3 Pt 1, pp. C693-701, (1998) (PubMed).

Friederich, Vancompernolle, Huet, Goethals, Finidori, Vandekerckhove, Louvard: "An actin-binding site containing a conserved motif of charged amino acid residues is essential for the morphogenic effect of villin." in: Cell, Vol. 70, Issue 1, pp. 81-92, (1992) (PubMed).

Friederich, Pringault, Arpin, Louvard: "From the structure to the function of villin, an actin-binding protein of the brush border." in: BioEssays : news and reviews in molecular, cellular and developmental biology, Vol. 12, Issue 9, pp. 403-8, (1991) (PubMed).

Details zu Villin 1

Target: Villin 1 (VIL1)

Andere Bezeichnung: Villin

Hintergrund: The isolated intestinal microvillus cytoskeleton (core) consists of four major proteins: actin, villin, fimbrin, and brush border myosin-I. These proteins can assemble in vitro into structures resembling native microvillus cores. Of these components, villin, and brush border myosin-I show tissue-specific expression, so they may be involved in the morphogenesis of intestinal microvilli. Found in association with the microvillar actin bundles of the intestinal brush border, villin is a 95 kDa protein composed of two very similar domains of approximately 44 kDa each, the core, and a C-terminal domain of 8 kDa, the headpiece. The core has been shown to contain villin's Ca[2+] regulated capping, nucleating, and severing activities, but it cannot induce the formation of microfilament bundles without the headpiece. Villin is a useful differentiation marker of early embryogenesis and may be useful in diagnosis and follow-up of colorectal cancers. It has been demonstrated that villin is necessary for both the cytoskeletal and membrane protein organization of a functional brush border. This antibody is routinely tested by western blot analysis.

Molekulargewicht: 95 kDa

Pathways: EGFR Signaling Pathway, Regulation of Actin Filament Polymerization